1e8o: Difference between revisions

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CORE OF THE ALU DOMAIN OF THE MAMMALIAN SRP

OverviewOverview

The Alu domain of the mammalian signal recognition particle (SRP) comprises the heterodimer of proteins SRP9 and SRP14 bound to the 5' and 3' terminal sequences of SRP RNA. It retards the ribosomal elongation of signal-peptide-containing proteins before their engagement with the translocation machinery in the endoplasmic reticulum. Here we report two crystal structures of the heterodimer SRP9/14 bound either to the 5' domain or to a construct containing both 5' and 3' domains. We present a model of the complete Alu domain that is consistent with extensive biochemical data. SRP9/14 binds strongly to the conserved core of the 5' domain, which forms a U-turn connecting two helical stacks. Reversible docking of the more weakly bound 3' domain might be functionally important in the mechanism of translational regulation. The Alu domain structure is probably conserved in other cytoplasmic ribonucleoprotein particles and retroposition intermediates containing SRP9/14-bound RNAs transcribed from Alu repeats or related elements in genomic DNA.

About this StructureAbout this Structure

1E8O is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structure and assembly of the Alu domain of the mammalian signal recognition particle., Weichenrieder O, Wild K, Strub K, Cusack S, Nature. 2000 Nov 9;408(6809):167-73. PMID:11089964

Page seeded by OCA on Thu Mar 20 10:51:44 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1e8o.jpg|left|200px]]<br /><applet load="1e8o" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1e8o.jpg|left|200px]]
-caption="1e8o, resolution 3.20&Aring;" />
-'''CORE OF THE ALU DOMAIN OF THE MAMMALIAN SRP'''<br />
+ {{Structure
+|PDB= 1e8o |SIZE=350|CAPTION= <scene name='initialview01'>1e8o</scene>, resolution 3.20&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''CORE OF THE ALU DOMAIN OF THE MAMMALIAN SRP'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-E8O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E8O OCA].
+E8O is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E8O OCA].
 ==Reference==
-Structure and assembly of the Alu domain of the mammalian signal recognition particle., Weichenrieder O, Wild K, Strub K, Cusack S, Nature. 2000 Nov 9;408(6809):167-73. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11089964 11089964]
+Structure and assembly of the Alu domain of the mammalian signal recognition particle., Weichenrieder O, Wild K, Strub K, Cusack S, Nature. 2000 Nov 9;408(6809):167-73. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11089964 11089964]
 [[Category: Homo sapiens]]
 [[Category: Protein complex]]
@@ Line 24: / Line 33: @@
 [[Category: translational control]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:25:06 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:51:44 2008''