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RUBREDOXIN OXYGEN:OXIDOREDUCTASE (ROO) FROM ANAEROBE DESULFOVIBRIO GIGAS

OverviewOverview

Desulfovibrio gigas is a strict anaerobe that contains a well-characterized metabolic pathway that enables it to survive transient contacts with oxygen. The terminal enzyme in this pathway, rubredoxin:oxygen oxidoreductase (ROO) reduces oxygen to water in a direct and safe way. The 2.5 A resolution crystal structure of ROO shows that each monomer of this homodimeric enzyme consists of a novel combination of two domains, a flavodoxin-like domain and a Zn-beta-lactamase-like domain that contains a di-iron center for dioxygen reduction. This is the first structure of a member of a superfamily of enzymes widespread in strict and facultative anaerobes, indicating its broad physiological significance.

About this StructureAbout this Structure

1E5D is a Single protein structure of sequence from Desulfovibrio gigas. Full crystallographic information is available from OCA.

ReferenceReference

Structure of a dioxygen reduction enzyme from Desulfovibrio gigas., Frazao C, Silva G, Gomes CM, Matias P, Coelho R, Sieker L, Macedo S, Liu MY, Oliveira S, Teixeira M, Xavier AV, Rodrigues-Pousada C, Carrondo MA, Le Gall J, Nat Struct Biol. 2000 Nov;7(11):1041-5. PMID:11062560

Page seeded by OCA on Thu Mar 20 10:49:58 2008

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OCA

@@ Line 1: / Line 1: @@
-[[Image:1e5d.jpg|left|200px]]<br /><applet load="1e5d" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1e5d.jpg|left|200px]]
-caption="1e5d, resolution 2.50&Aring;" />
-'''RUBREDOXIN OXYGEN:OXIDOREDUCTASE (ROO) FROM ANAEROBE DESULFOVIBRIO GIGAS'''<br />
+ {{Structure
+|PDB= 1e5d |SIZE=350|CAPTION= <scene name='initialview01'>1e5d</scene>, resolution 2.50&Aring;
+|SITE= <scene name='pdbsite=FEA:Diiron+Redox+Catalytic+Site+For+Direct+Dioxygen+To+Water+...'>FEA</scene>, <scene name='pdbsite=FEB:Diiron+Redox+Catalytic+Site+For+Direct+Dioxygen+To+Water+...'>FEB</scene>, <scene name='pdbsite=FMA:Fmn+Binding+Site+For+Chain+A'>FMA</scene> and <scene name='pdbsite=FMB:Fmn+Binding+Site+For+Chain+B'>FMB</scene>
+|LIGAND= <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=FEO:MU-OXO-DIIRON'>FEO</scene> and <scene name='pdbligand=OXY:OXYGEN MOLECULE'>OXY</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''RUBREDOXIN OXYGEN:OXIDOREDUCTASE (ROO) FROM ANAEROBE DESULFOVIBRIO GIGAS'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-E5D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_gigas Desulfovibrio gigas] with <scene name='pdbligand=FMN:'>FMN</scene>, <scene name='pdbligand=FEO:'>FEO</scene> and <scene name='pdbligand=OXY:'>OXY</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=FEA:Diiron+Redox+Catalytic+Site+For+Direct+Dioxygen+To+Water+...'>FEA</scene>, <scene name='pdbsite=FEB:Diiron+Redox+Catalytic+Site+For+Direct+Dioxygen+To+Water+...'>FEB</scene>, <scene name='pdbsite=FMA:Fmn+Binding+Site+For+Chain+A'>FMA</scene> and <scene name='pdbsite=FMB:Fmn+Binding+Site+For+Chain+B'>FMB</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E5D OCA].
+E5D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_gigas Desulfovibrio gigas]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E5D OCA].
 ==Reference==
-Structure of a dioxygen reduction enzyme from Desulfovibrio gigas., Frazao C, Silva G, Gomes CM, Matias P, Coelho R, Sieker L, Macedo S, Liu MY, Oliveira S, Teixeira M, Xavier AV, Rodrigues-Pousada C, Carrondo MA, Le Gall J, Nat Struct Biol. 2000 Nov;7(11):1041-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11062560 11062560]
+Structure of a dioxygen reduction enzyme from Desulfovibrio gigas., Frazao C, Silva G, Gomes CM, Matias P, Coelho R, Sieker L, Macedo S, Liu MY, Oliveira S, Teixeira M, Xavier AV, Rodrigues-Pousada C, Carrondo MA, Le Gall J, Nat Struct Biol. 2000 Nov;7(11):1041-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11062560 11062560]
 [[Category: Desulfovibrio gigas]]
 [[Category: Single protein]]
@@ Line 31: / Line 40: @@
 [[Category: OXY]]
 [[Category: diiron-centre]]
-[[Category: flavoproteins]]
+[[Category: flavoprotein]]
 [[Category: lactamase-fold]]
 [[Category: oxidoreductase]]
 [[Category: oxygenreductase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:24:00 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:49:58 2008''