1dyl: Difference between revisions

Revision as of 11:46, 20 March 2008

, resolution 9.Å

Coordinates:

9 ANGSTROM RESOLUTION CRYO-EM RECONSTRUCTION STRUCTURE OF SEMLIKI FOREST VIRUS (SFV) AND FITTING OF THE CAPSID PROTEIN STRUCTURE IN THE EM DENSITY

OverviewOverview

Semliki Forest virus serves as a paradigm for membrane fusion and assembly. Our icosahedral reconstruction combined 5276 particle images from 48 cryo-electron micrographs and determined the virion structure to 9 A resolution. The improved resolution of this map reveals an N-terminal arm linking capsid subunits and defines the spike-capsid interaction sites. It illustrates the paired helical nature of the transmembrane segments and the elongated structures connecting them to the spike projecting domains. A 10 A diameter density in the fusion protein lines the cavity at the center of the spike. These clearly visible features combine with the variation in order between the layers to provide a framework for understanding the structural changes during the life cycle of an enveloped virus.

About this StructureAbout this Structure

1DYL is a Single protein structure of sequence from Semliki forest virus. Full crystallographic information is available from OCA.

ReferenceReference

Cryo-electron microscopy reveals the functional organization of an enveloped virus, Semliki Forest virus., Mancini EJ, Clarke M, Gowen BE, Rutten T, Fuller SD, Mol Cell. 2000 Feb;5(2):255-66. PMID:10882067

Page seeded by OCA on Thu Mar 20 10:46:36 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1dyl.gif|left|200px]]<br /><applet load="1dyl" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1dyl.gif|left|200px]]
-caption="1dyl, resolution 9.&Aring;" />
-'''9 ANGSTROM RESOLUTION CRYO-EM RECONSTRUCTION STRUCTURE OF SEMLIKI FOREST VIRUS (SFV) AND FITTING OF THE CAPSID PROTEIN STRUCTURE IN THE EM DENSITY'''<br />
+ {{Structure
+|PDB= 1dyl |SIZE=350|CAPTION= <scene name='initialview01'>1dyl</scene>, resolution 9.&Aring;
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''9 ANGSTROM RESOLUTION CRYO-EM RECONSTRUCTION STRUCTURE OF SEMLIKI FOREST VIRUS (SFV) AND FITTING OF THE CAPSID PROTEIN STRUCTURE IN THE EM DENSITY'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-DYL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Semliki_forest_virus Semliki forest virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DYL OCA].
+DYL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Semliki_forest_virus Semliki forest virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DYL OCA].
 ==Reference==
-Cryo-electron microscopy reveals the functional organization of an enveloped virus, Semliki Forest virus., Mancini EJ, Clarke M, Gowen BE, Rutten T, Fuller SD, Mol Cell. 2000 Feb;5(2):255-66. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10882067 10882067]
+Cryo-electron microscopy reveals the functional organization of an enveloped virus, Semliki Forest virus., Mancini EJ, Clarke M, Gowen BE, Rutten T, Fuller SD, Mol Cell. 2000 Feb;5(2):255-66. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10882067 10882067]
 [[Category: Semliki forest virus]]
 [[Category: Single protein]]
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 [[Category: sfv]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:21:52 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:46:36 2008''