1dud: Difference between revisions
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[[Image:1dud.gif|left|200px]] | [[Image:1dud.gif|left|200px]] | ||
'''DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDE HYDROLASE (D-UTPASE) COMPLEXED WITH THE SUBSTRATE ANALOGUE DEOXYURIDINE 5'-DIPHOSPHATE (D-UDP)''' | {{Structure | ||
|PDB= 1dud |SIZE=350|CAPTION= <scene name='initialview01'>1dud</scene>, resolution 2.3Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=DUD:DEOXYURIDINE-5'-DIPHOSPHATE'>DUD</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/dUTP_diphosphatase dUTP diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.23 3.6.1.23] | |||
|GENE= | |||
}} | |||
'''DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDE HYDROLASE (D-UTPASE) COMPLEXED WITH THE SUBSTRATE ANALOGUE DEOXYURIDINE 5'-DIPHOSPHATE (D-UDP)''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1DUD is a [ | 1DUD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DUD OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of the Escherichia coli dUTPase in complex with a substrate analogue (dUDP)., Larsson G, Svensson LA, Nyman PO, Nat Struct Biol. 1996 Jun;3(6):532-8. PMID:[http:// | Crystal structure of the Escherichia coli dUTPase in complex with a substrate analogue (dUDP)., Larsson G, Svensson LA, Nyman PO, Nat Struct Biol. 1996 Jun;3(6):532-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8646539 8646539] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: substrate analogue]] | [[Category: substrate analogue]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:44:27 2008'' | ||
Revision as of 11:44, 20 March 2008
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| , resolution 2.3Å | |||||||
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| Ligands: | |||||||
| Activity: | dUTP diphosphatase, with EC number 3.6.1.23 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDE HYDROLASE (D-UTPASE) COMPLEXED WITH THE SUBSTRATE ANALOGUE DEOXYURIDINE 5'-DIPHOSPHATE (D-UDP)
OverviewOverview
We have determined the structure of the homotrimeric dUTPase from Escherichia coli, completed with an inhibitor and substrate analogue, dUDP. Three molecules of dUDP are found symmetrically bound per trimer, each in a shallow cleft between adjacent subunits, interacting with evolutionary conserved residues. The interactions of the uracil ring and the deoxypentose with the protein are consistent with the high specificity of the enzyme with respect to these groups. The positions of the two phosphate groups and adjacent water molecules are discussed in relation to the mechanism and kinetics of catalysis. The role that dUTPase plays in DNA metabolism makes the enzyme a potential target for chemotherapeutic drugs: the results presented here will aid in the design and development of inhibitory compounds.
About this StructureAbout this Structure
1DUD is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the Escherichia coli dUTPase in complex with a substrate analogue (dUDP)., Larsson G, Svensson LA, Nyman PO, Nat Struct Biol. 1996 Jun;3(6):532-8. PMID:8646539
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