1diq: Difference between revisions
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[[Image:1diq.gif|left|200px]] | [[Image:1diq.gif|left|200px]] | ||
'''CRYSTAL STRUCTURE OF P-CRESOL METHYLHYDROXYLASE WITH SUBSTRATE BOUND''' | {{Structure | ||
|PDB= 1diq |SIZE=350|CAPTION= <scene name='initialview01'>1diq</scene>, resolution 2.75Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=PCR:P-CRESOL'>PCR</scene> and <scene name='pdbligand=HEM:PROTOPORPHYRIN IX CONTAINING FE'>HEM</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/4-cresol_dehydrogenase_(hydroxylating) 4-cresol dehydrogenase (hydroxylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.99.1 1.17.99.1] | |||
|GENE= | |||
}} | |||
'''CRYSTAL STRUCTURE OF P-CRESOL METHYLHYDROXYLASE WITH SUBSTRATE BOUND''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1DIQ is a [ | 1DIQ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DIQ OCA]. | ||
==Reference== | ==Reference== | ||
Structures of the flavocytochrome p-cresol methylhydroxylase and its enzyme-substrate complex: gated substrate entry and proton relays support the proposed catalytic mechanism., Cunane LM, Chen ZW, Shamala N, Mathews FS, Cronin CN, McIntire WS, J Mol Biol. 2000 Jan 14;295(2):357-74. PMID:[http:// | Structures of the flavocytochrome p-cresol methylhydroxylase and its enzyme-substrate complex: gated substrate entry and proton relays support the proposed catalytic mechanism., Cunane LM, Chen ZW, Shamala N, Mathews FS, Cronin CN, McIntire WS, J Mol Biol. 2000 Jan 14;295(2):357-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10623531 10623531] | ||
[[Category: 4-cresol dehydrogenase (hydroxylating)]] | [[Category: 4-cresol dehydrogenase (hydroxylating)]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: p-cresol]] | [[Category: p-cresol]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:39:02 2008'' | ||
Revision as of 11:39, 20 March 2008
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| , resolution 2.75Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , and | ||||||
| Activity: | 4-cresol dehydrogenase (hydroxylating), with EC number 1.17.99.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF P-CRESOL METHYLHYDROXYLASE WITH SUBSTRATE BOUND
OverviewOverview
The degradation of the toxic phenol p-cresol by Pseudomonas bacteria occurs by way of the protocatechuate metabolic pathway. The first enzyme in this pathway, p-cresol methylhydroxylase (PCMH), is a flavocytochrome c. The enzyme first catalyzes the oxidation of p-cresol to p-hydroxybenzyl alcohol, utilizing one atom of oxygen derived from water, and yielding one molecule of reduced FAD. The reducing electron equivalents are then passed one at a time from the flavin cofactor to the heme cofactor by intramolecular electron transfer, and subsequently to cytochrome oxidase within the periplasmic membrane via one or more soluble electron carrier proteins. The product, p-hydroxybenzyl alcohol, can also be oxidized by PCMH to yield p-hydroxybenzaldehyde. The fully refined X-ray crystal structure of PCMH in the native state has been obtained at 2. 5 A resolution on the basis of the gene sequence. The structure of the enzyme-substrate complex has also been refined, at 2.75 A resolution, and reveals significant conformational changes in the active site upon substrate binding. The active site for substrate oxidation is deeply buried in the interior of the PCMH molecule. A route for substrate access to the site has been identified and is shown to be governed by a swinging-gate mechanism. Two possible proton transfer pathways, that may assist in activating the substrate for nucleophilic attack and in removal of protons generated during the reaction, have been revealed. Hydrogen bonding interactions between the flavoprotein and cytochrome subunits that stabilize the intramolecular complex and may contribute to the electron transfer process have been identified.
About this StructureAbout this Structure
1DIQ is a Protein complex structure of sequences from Pseudomonas putida. Full crystallographic information is available from OCA.
ReferenceReference
Structures of the flavocytochrome p-cresol methylhydroxylase and its enzyme-substrate complex: gated substrate entry and proton relays support the proposed catalytic mechanism., Cunane LM, Chen ZW, Shamala N, Mathews FS, Cronin CN, McIntire WS, J Mol Biol. 2000 Jan 14;295(2):357-74. PMID:10623531
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