1dei: Difference between revisions

Revision as of 11:37, 20 March 2008

, resolution 1.6Å

Coordinates:

DESHEPTAPEPTIDE (B24-B30) INSULIN

OverviewOverview

The crystal structure of desheptapeptide (B24-B30) insulin (DHPI), a virtually inactive analog of insulin, was determined at 1.6 A resolution. In the refined structure model, DHPI retains three alpha-helices (A1-A8, A12-A18, and B9-B19) as its structural framework, while great conformational changes occur in the N and C termini of B-chain. The beta-turn, which lies in B20-B30 in insulin and insulin analogs with high potency, no longer exists in DHPI. Relative motion is observed among the three alpha-helices, each as a rigid functional group. In contrast, a region covering B5-B6 and A6-A11 exhibits a relatively stable conformation. We interpret our results as identifying: (i) the importance of beta-turn in determining the receptor-binding potency of insulin and (ii) a leading role of PheB24 in maintaining the beta-turn structure.

About this StructureAbout this Structure

1DEI is a Protein complex structure of sequences from Sus scrofa. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of desheptapeptide(B24-B30)insulin at 1.6 A resolution: implications for receptor binding., Bao SJ, Xie DL, Zhang JP, Chang WR, Liang DC, Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):2975-80. PMID:9096331

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OCA

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-[[Image:1dei.jpg|left|200px]]<br /><applet load="1dei" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1dei.jpg|left|200px]]
-caption="1dei, resolution 1.6&Aring;" />
-'''DESHEPTAPEPTIDE (B24-B30) INSULIN'''<br />
+ {{Structure
+|PDB= 1dei |SIZE=350|CAPTION= <scene name='initialview01'>1dei</scene>, resolution 1.6&Aring;
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''DESHEPTAPEPTIDE (B24-B30) INSULIN'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-DEI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DEI OCA].
+DEI is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DEI OCA].
 ==Reference==
-Crystal structure of desheptapeptide(B24-B30)insulin at 1.6 A resolution: implications for receptor binding., Bao SJ, Xie DL, Zhang JP, Chang WR, Liang DC, Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):2975-80. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9096331 9096331]
+Crystal structure of desheptapeptide(B24-B30)insulin at 1.6 A resolution: implications for receptor binding., Bao SJ, Xie DL, Zhang JP, Chang WR, Liang DC, Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):2975-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9096331 9096331]
 [[Category: Protein complex]]
 [[Category: Sus scrofa]]
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 [[Category: hormone]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:15:50 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:37:18 2008''