1da1: Difference between revisions
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[[Image:1da1.gif|left|200px]] | [[Image:1da1.gif|left|200px]] | ||
'''STRUCTURAL CHARACTERISATION OF THE BROMOURACIL-GUANINE BASE PAIR MISMATCH IN A Z-DNA FRAGMENT''' | {{Structure | ||
|PDB= 1da1 |SIZE=350|CAPTION= <scene name='initialview01'>1da1</scene>, resolution 2.250Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''STRUCTURAL CHARACTERISATION OF THE BROMOURACIL-GUANINE BASE PAIR MISMATCH IN A Z-DNA FRAGMENT''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1DA1 is a [ | 1DA1 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DA1 OCA]. | ||
==Reference== | ==Reference== | ||
Structural characterisation of the bromouracil.guanine base pair mismatch in a Z-DNA fragment., Brown T, Kneale G, Hunter WN, Kennard O, Nucleic Acids Res. 1986 Feb 25;14(4):1801-9. PMID:[http:// | Structural characterisation of the bromouracil.guanine base pair mismatch in a Z-DNA fragment., Brown T, Kneale G, Hunter WN, Kennard O, Nucleic Acids Res. 1986 Feb 25;14(4):1801-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/3951996 3951996] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Brown, T.]] | [[Category: Brown, T.]] | ||
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[[Category: z-dna]] | [[Category: z-dna]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:35:11 2008'' | ||
Revision as of 11:35, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURAL CHARACTERISATION OF THE BROMOURACIL-GUANINE BASE PAIR MISMATCH IN A Z-DNA FRAGMENT
OverviewOverview
The deoxyoligonucleotide d(BrU-G-C-G-C-G) was crystallised at pH 8.2 and its structure analysed by X-ray diffraction. The unit cell, of dimensions a = 17.94, b = 30.85, c = 49.94A contains four DNA duplexes in space group P2(1)2(1)2(1). The duplexes are in the Z conformation, with four Watson-Crick G.C base pairs and two BrU.G base pairs. The structure was refined to an R factor of 0.16 at a resolution of 2.2A with 64 solvent molecules located. The BrU.G base pair mismatch is of the wobble type, with both bases in the major tautomer form and hydrogen bonds linking 0-2 of BrU with N-1 of G and N3 of BrU with 0-6 of G. There is no indication of the presence of ionised base pairs, in spite of the high pH of crystallisation. The results are discussed in terms of the mutagenic properties of 5- bromouracil.
About this StructureAbout this Structure
1DA1 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
ReferenceReference
Structural characterisation of the bromouracil.guanine base pair mismatch in a Z-DNA fragment., Brown T, Kneale G, Hunter WN, Kennard O, Nucleic Acids Res. 1986 Feb 25;14(4):1801-9. PMID:3951996
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