1d3y: Difference between revisions
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[[Image:1d3y.gif|left|200px]] | [[Image:1d3y.gif|left|200px]] | ||
'''STRUCTURE OF THE DNA TOPOISOMERASE VI A SUBUNIT''' | {{Structure | ||
|PDB= 1d3y |SIZE=350|CAPTION= <scene name='initialview01'>1d3y</scene>, resolution 2.0Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> and <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/DNA_topoisomerase_(ATP-hydrolyzing) DNA topoisomerase (ATP-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.3 5.99.1.3] | |||
|GENE= | |||
}} | |||
'''STRUCTURE OF THE DNA TOPOISOMERASE VI A SUBUNIT''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1D3Y is a [ | 1D3Y is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D3Y OCA]. | ||
==Reference== | ==Reference== | ||
Structure and function of an archaeal topoisomerase VI subunit with homology to the meiotic recombination factor Spo11., Nichols MD, DeAngelis K, Keck JL, Berger JM, EMBO J. 1999 Nov 1;18(21):6177-88. PMID:[http:// | Structure and function of an archaeal topoisomerase VI subunit with homology to the meiotic recombination factor Spo11., Nichols MD, DeAngelis K, Keck JL, Berger JM, EMBO J. 1999 Nov 1;18(21):6177-88. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10545127 10545127] | ||
[[Category: DNA topoisomerase (ATP-hydrolyzing)]] | [[Category: DNA topoisomerase (ATP-hydrolyzing)]] | ||
[[Category: Methanocaldococcus jannaschii]] | [[Category: Methanocaldococcus jannaschii]] | ||
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[[Category: topoisomerase vi]] | [[Category: topoisomerase vi]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:32:34 2008'' | ||
Revision as of 11:32, 20 March 2008
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| , resolution 2.0Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Activity: | DNA topoisomerase (ATP-hydrolyzing), with EC number 5.99.1.3 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF THE DNA TOPOISOMERASE VI A SUBUNIT
OverviewOverview
In all organisms, type II DNA topoisomerases are essential for untangling chromosomal DNA. We have determined the structure of the DNA-binding core of the Methanococcus jannaschii DNA topoisomerase VI A subunit at 2.0 A resolution. The overall structure of this subunit is unique, demonstrating that archaeal type II enzymes are distinct from other type II topoisomerases. However, the core structure contains a pair of domains that are also found in type IA and classic type II topoisomerases. Together, these regions may form the basis of a DNA cleavage mechanism shared among these enzymes. The core A subunit is a dimer that contains a deep groove that spans both protomers. The dimer architecture suggests that DNA is bound in the groove, across the A subunit interface, and that the two monomers separate during DNA transport. The A subunit of topoisomerase VI is homologous to the meiotic recombination factor, Spo11, and this structure can serve as a template for probing Spo11 function in eukaryotes.
About this StructureAbout this Structure
1D3Y is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.
ReferenceReference
Structure and function of an archaeal topoisomerase VI subunit with homology to the meiotic recombination factor Spo11., Nichols MD, DeAngelis K, Keck JL, Berger JM, EMBO J. 1999 Nov 1;18(21):6177-88. PMID:10545127
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