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''' | ==Crystal Structure Determination of Pseudomonas stutzeri endoglucanase Cel5A using a Twinned Data Set== | ||
<StructureSection load='4lx4' size='340' side='right' caption='[[4lx4]], [[Resolution|resolution]] 1.56Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4lx4]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LX4 FirstGlance]. <br> | |||
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene><br> | |||
<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr> | |||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lx4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4lx4 RCSB], [http://www.ebi.ac.uk/pdbsum/4lx4 PDBsum]</span></td></tr> | |||
<table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
In this study, the mining of an Antarctic soil sample by functional metagenomics allowed the isolation of a cold-adapted protein (RBcel1) that hydrolyzes only carboxymethyl cellulose. The new enzyme is related to family 5 of the glycosyl hydrolase (GH5) protein from Pseudomonas stutzeri (Pst_2494) and does not possess a carbohydrate-binding domain. The protein was produced and purified to homogeneity. RBcel1 displayed an endoglucanase activity, producing cellobiose and cellotriose, using carboxymethyl cellulose as a substrate. Moreover, the study of pH and the thermal dependence of the hydrolytic activity shows that RBcel1 was active from pH 6 to pH 9 and remained significantly active when temperature decreased (18% of activity at 10 degrees C). It is interesting that RBcel1 was able to synthetize non-reticulated cellulose using cellobiose as a substrate. Moreover, by a combination of bioinformatics and enzyme analysis, the physiological relevance of the RBcel1 protein and its mesophilic homologous Pst_2494 protein from P. stutzeri, A1501, was established as the key enzymes involved in the production of cellulose by bacteria. In addition, RBcel1 and Pst_2494 are the two primary enzymes belonging to the GH5 family involved in this process. | |||
Insights into bacterial cellulose biosynthesis by functional metagenomics on Antarctic soil samples.,Berlemont R, Delsaute M, Pipers D, D'Amico S, Feller G, Galleni M, Power P ISME J. 2009 Sep;3(9):1070-81. doi: 10.1038/ismej.2009.48. Epub 2009 May 21. PMID:19458657<ref>PMID:19458657</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Cellulase]] | |||
[[Category: Bauvois, C.]] | |||
[[Category: Berlemont, R.]] | |||
[[Category: Delsaute, M.]] | |||
[[Category: Dutoit, R.]] | |||
[[Category: Elder, D Van.]] | |||
[[Category: Galleni, M.]] | |||
[[Category: 4-endoglucanase]] | |||
[[Category: Beta-1]] | |||
[[Category: Cellulase]] | |||
[[Category: Glycosyl hydrolase family 5]] | |||
[[Category: Hydrolase]] | |||
[[Category: Tim barrel]] | |||
Revision as of 10:36, 30 July 2014
Crystal Structure Determination of Pseudomonas stutzeri endoglucanase Cel5A using a Twinned Data SetCrystal Structure Determination of Pseudomonas stutzeri endoglucanase Cel5A using a Twinned Data Set
Structural highlights
Publication Abstract from PubMedIn this study, the mining of an Antarctic soil sample by functional metagenomics allowed the isolation of a cold-adapted protein (RBcel1) that hydrolyzes only carboxymethyl cellulose. The new enzyme is related to family 5 of the glycosyl hydrolase (GH5) protein from Pseudomonas stutzeri (Pst_2494) and does not possess a carbohydrate-binding domain. The protein was produced and purified to homogeneity. RBcel1 displayed an endoglucanase activity, producing cellobiose and cellotriose, using carboxymethyl cellulose as a substrate. Moreover, the study of pH and the thermal dependence of the hydrolytic activity shows that RBcel1 was active from pH 6 to pH 9 and remained significantly active when temperature decreased (18% of activity at 10 degrees C). It is interesting that RBcel1 was able to synthetize non-reticulated cellulose using cellobiose as a substrate. Moreover, by a combination of bioinformatics and enzyme analysis, the physiological relevance of the RBcel1 protein and its mesophilic homologous Pst_2494 protein from P. stutzeri, A1501, was established as the key enzymes involved in the production of cellulose by bacteria. In addition, RBcel1 and Pst_2494 are the two primary enzymes belonging to the GH5 family involved in this process. Insights into bacterial cellulose biosynthesis by functional metagenomics on Antarctic soil samples.,Berlemont R, Delsaute M, Pipers D, D'Amico S, Feller G, Galleni M, Power P ISME J. 2009 Sep;3(9):1070-81. doi: 10.1038/ismej.2009.48. Epub 2009 May 21. PMID:19458657[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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