1d39: Difference between revisions

Revision as of 11:32, 20 March 2008

, resolution 1.200Å

Ligands:

and

Coordinates:

COVALENT MODIFICATION OF GUANINE BASES IN DOUBLE STRANDED DNA: THE 1.2 ANGSTROMS Z-DNA STRUCTURE OF D(CGCGCG) IN THE PRESENCE OF CUCL2

OverviewOverview

We have solved the single crystal structure to 1.2-A resolution of the Z-DNA sequence d(CGCGCG) soaked with copper(II) chloride. This structure allows us to elucidate the structural properties of copper in a model that mimics a physiologically relevant environment. A copper(II) cation was observed to form a covalent coordinate bond to N-7 of each guanine base along the hexamer duplex. The occurrence of copper bound at each site was dependent on the exposure of the bases and the packing of the hexamers in the crystal. The copper at the highest occupied site was observed to form a regular octahedral complex, with four water ligands in the equatorial plane and a fifth water along with N-7 of the purine base at the axial positions. All other copper complexes appear to be variations of this structure. By using the octahedral complex as the prototype for copper(II) binding to guanine bases in the Z-DNA crystal, model structures were built showing that duplex B-DNA can accommodate octahedral copper(II) complexes at the guanine bases as well as copper complexes bridged at adjacent guanine residues by a reactive dioxygen species. The increased susceptibility to oxidative DNA cleavage induced by copper(II) ions in solution of the bases located 5' to one or more adjacent guanine residues can thus be explained in terms of the cation and DNA structures described by these models.

About this StructureAbout this Structure

1D39 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

ReferenceReference

Covalent modification of guanine bases in double-stranded DNA. The 1.2-A Z-DNA structure of d(CGCGCG) in the presence of CuCl2., Kagawa TF, Geierstanger BH, Wang AH, Ho PS, J Biol Chem. 1991 Oct 25;266(30):20175-84. PMID:1939078

Page seeded by OCA on Thu Mar 20 10:32:17 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1d39.gif|left|200px]]<br /><applet load="1d39" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1d39.gif|left|200px]]
-caption="1d39, resolution 1.200&Aring;" />
-'''COVALENT MODIFICATION OF GUANINE BASES IN DOUBLE STRANDED DNA: THE 1.2 ANGSTROMS Z-DNA STRUCTURE OF D(CGCGCG) IN THE PRESENCE OF CUCL2'''<br />
+ {{Structure
+|PDB= 1d39 |SIZE=350|CAPTION= <scene name='initialview01'>1d39</scene>, resolution 1.200&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene> and <scene name='pdbligand=NA:SODIUM ION'>NA</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''COVALENT MODIFICATION OF GUANINE BASES IN DOUBLE STRANDED DNA: THE 1.2 ANGSTROMS Z-DNA STRUCTURE OF D(CGCGCG) IN THE PRESENCE OF CUCL2'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-D39 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CU:'>CU</scene> and <scene name='pdbligand=NA:'>NA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D39 OCA].
+D39 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D39 OCA].
 ==Reference==
-Covalent modification of guanine bases in double-stranded DNA. The 1.2-A Z-DNA structure of d(CGCGCG) in the presence of CuCl2., Kagawa TF, Geierstanger BH, Wang AH, Ho PS, J Biol Chem. 1991 Oct 25;266(30):20175-84. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1939078 1939078]
+Covalent modification of guanine bases in double-stranded DNA. The 1.2-A Z-DNA structure of d(CGCGCG) in the presence of CuCl2., Kagawa TF, Geierstanger BH, Wang AH, Ho PS, J Biol Chem. 1991 Oct 25;266(30):20175-84. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1939078 1939078]
 [[Category: Protein complex]]
 [[Category: Geierstanger, B H.]]
@@ Line 22: / Line 31: @@
 [[Category: z-dna]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:12:29 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:32:17 2008''