4pm3: Difference between revisions
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''' | ==Structure of the double-stranded DNA binding type IV secretion protein TraN from Enterococcus== | ||
<StructureSection load='4pm3' size='340' side='right' caption='[[4pm3]], [[Resolution|resolution]] 1.80Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4pm3]] is a 2 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4hh7 4hh7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PM3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PM3 FirstGlance]. <br> | |||
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=PT:PLATINUM+(II)+ION'>PT</scene><br> | |||
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4hh7|4hh7]]</td></tr> | |||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pm3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pm3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pm3 RCSB], [http://www.ebi.ac.uk/pdbsum/4pm3 PDBsum]</span></td></tr> | |||
<table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Conjugative plasmid transfer is the most important route for the spread of resistance and virulence genes among bacteria. Consequently, bacteria carrying conjugative plasmids are a substantial threat to human health, especially hospitalized patients. Whilst detailed information about the process has been obtained for Gram-negative type-4 secretion systems, little is known about the corresponding mechanisms in Gram-positive (G+) bacteria. The successful purification and crystallization of the putative transfer protein TraN from the G+ conjugative model plasmid pIP501 of Enterococcus faecalis are presented. Native crystals diffracted to 1.8 A resolution on a synchrotron beamline. The crystals belonged to space group P2(1), with unit-cell parameters a=32.88, b=54.94, c=57.71 A, beta=91.89 degrees and two molecules per asymmetric unit. | |||
Crystallization and first data collection of the putative transfer protein TraN from the Gram-positive conjugative plasmid pIP501.,Goessweiner-Mohr N, Fercher C, Abajy MY, Grohmann E, Keller W Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Nov 1;68(Pt 11):1402-5., doi: 10.1107/S174430911204184X. Epub 2012 Oct 30. PMID:23143259<ref>PMID:23143259</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Goessweiner-Mohr, N.]] | |||
[[Category: Keller, W.]] | |||
[[Category: Dna binding protein]] | |||
[[Category: Internal dimer]] | |||
[[Category: Isomerase]] | |||
[[Category: Type iv secretion]] | |||
Revision as of 10:31, 30 July 2014
Structure of the double-stranded DNA binding type IV secretion protein TraN from EnterococcusStructure of the double-stranded DNA binding type IV secretion protein TraN from Enterococcus
Structural highlights
Publication Abstract from PubMedConjugative plasmid transfer is the most important route for the spread of resistance and virulence genes among bacteria. Consequently, bacteria carrying conjugative plasmids are a substantial threat to human health, especially hospitalized patients. Whilst detailed information about the process has been obtained for Gram-negative type-4 secretion systems, little is known about the corresponding mechanisms in Gram-positive (G+) bacteria. The successful purification and crystallization of the putative transfer protein TraN from the G+ conjugative model plasmid pIP501 of Enterococcus faecalis are presented. Native crystals diffracted to 1.8 A resolution on a synchrotron beamline. The crystals belonged to space group P2(1), with unit-cell parameters a=32.88, b=54.94, c=57.71 A, beta=91.89 degrees and two molecules per asymmetric unit. Crystallization and first data collection of the putative transfer protein TraN from the Gram-positive conjugative plasmid pIP501.,Goessweiner-Mohr N, Fercher C, Abajy MY, Grohmann E, Keller W Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Nov 1;68(Pt 11):1402-5., doi: 10.1107/S174430911204184X. Epub 2012 Oct 30. PMID:23143259[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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