1cx0: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1cx0.gif|left|200px]] | [[Image:1cx0.gif|left|200px]] | ||
'''HEPATITIS DELTA VIRUS RIBOZYME''' | {{Structure | ||
|PDB= 1cx0 |SIZE=350|CAPTION= <scene name='initialview01'>1cx0</scene>, resolution 2.30Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''HEPATITIS DELTA VIRUS RIBOZYME''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1CX0 is a [ | 1CX0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Hepatitis_delta_virus Hepatitis delta virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CX0 OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of a hepatitis delta virus ribozyme., Ferre-D'Amare AR, Zhou K, Doudna JA, Nature. 1998 Oct 8;395(6702):567-74. PMID:[http:// | Crystal structure of a hepatitis delta virus ribozyme., Ferre-D'Amare AR, Zhou K, Doudna JA, Nature. 1998 Oct 8;395(6702):567-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9783582 9783582] | ||
[[Category: Hepatitis delta virus]] | [[Category: Hepatitis delta virus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 19: | Line 28: | ||
[[Category: nested double pseudoknot rna structure]] | [[Category: nested double pseudoknot rna structure]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:30:01 2008'' | ||
Revision as of 11:30, 20 March 2008
| |||||||
| , resolution 2.30Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
HEPATITIS DELTA VIRUS RIBOZYME
OverviewOverview
The self-cleaving ribozyme of the hepatitis delta virus (HDV) is the only catalytic RNA known to be required for the viability of a human pathogen. We obtained crystals of a 72-nucleotide, self-cleaved form of the genomic HDV ribozyme that diffract X-rays to 2.3 A resolution by engineering the RNA to bind a small, basic protein without affecting ribozyme activity. The co-crystal structure shows that the compact catalytic core comprises five helical segments connected as an intricate nested double pseudoknot. The 5'-hydroxyl leaving group resulting from the self-scission reaction is buried deep within an active-site cleft produced by juxtaposition of the helices and five strand-crossovers, and is surrounded by biochemically important backbone and base functional groups in a manner reminiscent of protein enzymes.
About this StructureAbout this Structure
1CX0 is a Single protein structure of sequence from Hepatitis delta virus. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of a hepatitis delta virus ribozyme., Ferre-D'Amare AR, Zhou K, Doudna JA, Nature. 1998 Oct 8;395(6702):567-74. PMID:9783582
Page seeded by OCA on Thu Mar 20 10:30:01 2008