4g0a: Difference between revisions
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[[ | ==Crystallographic Analysis of Rotavirus NSP2-RNA Complex Reveals Specific Recognition of 5'-GG Sequence for RTPase activity== | ||
<StructureSection load='4g0a' size='340' side='right' caption='[[4g0a]], [[Resolution|resolution]] 2.10Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4g0a]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Rotsr Rotsr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G0A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G0A FirstGlance]. <br> | |||
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br> | |||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g0a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g0a RCSB], [http://www.ebi.ac.uk/pdbsum/4g0a PDBsum]</span></td></tr> | |||
<table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Rotavirus non-structural protein NSP2, a functional octamer, is critical for the formation of viroplasms, which are exclusive sites for replication and packaging of segmented double-stranded RNA (dsRNA) rotavirus genome. As a component of replication intermediates, NSP2 is also implicated in various replication-related activities. In addition to sequence-independent single-stranded RNA binding and helix-destabilizing activities, NSP2 exhibits monomer-associated nucleoside and 5' RNA triphosphatase (NTPase/RTPase) activities which are mediated by a conserved H225 residue within a narrow enzymatic cleft. Lack of a 5' gamma-phosphate is a common feature of the (-)RNA of the packaged dsRNA segments in RV. Strikingly, all (-)RNAs (in group A RV) have a 5' GG dinucleotide sequence. As the only rotavirus protein with 5' RTPase activity, NSP2 is implicated in the removal of the gamma-phosphate from the rotavirus minus-strand RNA. To understand how NSP2, despite its sequence-independent RNA binding property, recognizes minus-strand RNA to hydrolyze the gamma-phosphate within the catalytic cleft, we determined a crystal structure of NSP2 in complex with the 5' consensus sequence of minus-strand rotavirus RNA. Our studies show that the 5' GG of the bound oligoribonucleotide interacts extensively with highly conserved residues in the NSP2 enzymatic cleft. While these residues provide GG-specific interactions, surface plasmon resonance studies suggest that the C-terminal helix and other basic residues outside the enzymatic cleft account for sequence-independent RNA binding of NSP2. A novel observation from our studies, which may have implications in viroplasm formation, is that the C-terminal helix of NSP2 exhibits two distinct conformations and engages in domain swapping interactions, which result in the formation of NSP2 octamer chains. | |||
Crystallographic Analysis of Rotavirus NSP2-RNA Complex Reveals Specific Recognition of 5' -GG Sequence for RTPase activity.,Hu L, Chow DC, Patton JT, Palzkill T, Estes MK, Prasad BV J Virol. 2012 Jul 18. PMID:22811529<ref>PMID:22811529</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
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</StructureSection> | |||
[[Category: Rotsr]] | |||
== | |||
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[[Category: | |||
[[Category: Hu, L.]] | [[Category: Hu, L.]] | ||
[[Category: Prasad, B V.V.]] | [[Category: Prasad, B V.V.]] | ||