Protein kinase Spk1: Difference between revisions
Michal Harel (talk | contribs) New page: <StructureSection load='2aot' size='340' side='right' caption='Structure of yeast Rad53 FHA1 domain complex with phosphothreonine peptide (PDB code 2aot).' scene=''> == Function == ... |
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<StructureSection load='2a0t' size='340' side='right' caption='Structure of yeast Rad53 FHA1 domain complex with phosphothreonine peptide (PDB code [[2a0t]]).' scene=''> | |||
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[[1g3g]], [[1j4o]] – yRad53 FHA1 domain (mutant) - NMR<br /> | [[1g3g]], [[1j4o]] – yRad53 FHA1 domain (mutant) - NMR<br /> | ||
[[1j4p]], [[1j4q]], [[1k3n]], [[1k3q]] – yRad53 FHA1 domain + Rad9 peptide - NMR<br /> | [[1j4p]], [[1j4q]], [[1k3n]], [[1k3q]] – yRad53 FHA1 domain + Rad9 peptide - NMR<br /> | ||
[[1g6g]], [[ | [[1g6g]], [[2a0t]], [[2jqi]] – yRad53 FHA1 domain + phosphothreonine peptide <br /> | ||
'''Rad53 FHA2 domain''' | '''Rad53 FHA2 domain''' | ||
Revision as of 13:10, 9 June 2014
<StructureSection load='2a0t' size='340' side='right' caption='Structure of yeast Rad53 FHA1 domain complex with phosphothreonine peptide (PDB code 2a0t).' scene=>
FunctionFunction
Protein kinase Spk1 (Rad53) is a serine/threonine protein kinase which phosphorylates proteins. Rad53 controls S-phase checkpoint and G1 and G2 DNA damage checkpoints. Rad53 phosphorylates proteins at serine, threonine and tyrosine residues.
DiseaseDisease
RelevanceRelevance
Structural highlightsStructural highlights
Rad53 contains phosphothreonine recognition domains: FHA1 at the N-terminal (residues 1-164) which selects for Asp at position +3 relative to phosphothreonine and FHA2 at the C-terminal (residues 573-730) which selects for Ile at position +3 relative to phosphothreonine. Two SCD - SQ/TQ-rich cluster domains – are flanking the kinase domain.
3D structures of protein kinase Spk13D structures of protein kinase Spk1
Updated on 09-June-2014
Rad53 FHA1 domain
1k3j – yRad53 FHA1 domain – yeast - NMR
1g3g, 1j4o – yRad53 FHA1 domain (mutant) - NMR
1j4p, 1j4q, 1k3n, 1k3q – yRad53 FHA1 domain + Rad9 peptide - NMR
1g6g, 2a0t, 2jqi – yRad53 FHA1 domain + phosphothreonine peptide
Rad53 FHA2 domain
1qu5, 1dmz, 1fhq – yRad53 FHA2 domain – yeast - NMR
1fhr, 1j4k, 1j4l, 1k2m, 1k2n – yRad53 FHA2 domain + Rad9 peptide - NMR
Rad53 kinase and SCD2 domains
4pdp – yRad53 kinase and SCD2 domain (mutant)
4pds – yRad53 kinase and SCD2 domain (mutant) + AMPPNP