1bc7: Difference between revisions

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File:1bc7.gif

, resolution 2.01Å

Gene:

SAP-1 RESIDUES 1-93 (Homo sapiens)

Coordinates:

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SERUM RESPONSE FACTOR ACCESSORY PROTEIN 1A (SAP-1)/DNA COMPLEX

OverviewOverview

SAP-1 is a member of the Ets transcription factors and cooperates with SRF protein to activate transcription of the c-fos protooncogene. The crystal structures of the conserved ETS domain of SAP-1 bound to DNA sequences from the E74 and c-fos promoters reveal that a set of conserved residues contact a GGA core DNA sequence. Discrimination for sequences outside this core is mediated by DNA contacts from conserved and nonconserved protein residues and sequence-dependent DNA structural properties characteristic of A-form DNA structure. Comparison with the related PU.1/DNA and GABPalpha/beta/DNA complexes provides general insights into DNA discrimination between Ets proteins. Modeling studies of a SAP-1/SRF/DNA complex suggest that SRF may modulate SAP-1 binding to DNA by interacting with its ETS domain.

About this StructureAbout this Structure

1BC7 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structures of SAP-1 bound to DNA targets from the E74 and c-fos promoters: insights into DNA sequence discrimination by Ets proteins., Mo Y, Vaessen B, Johnston K, Marmorstein R, Mol Cell. 1998 Aug;2(2):201-12. PMID:9734357

Page seeded by OCA on Thu Mar 20 10:09:02 2008

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OCA

@@ Line 1: / Line 1: @@
-[[Image:1bc7.gif|left|200px]]<br /><applet load="1bc7" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1bc7.gif|left|200px]]
-caption="1bc7, resolution 2.01&Aring;" />
-'''SERUM RESPONSE FACTOR ACCESSORY PROTEIN 1A (SAP-1)/DNA COMPLEX'''<br />
+ {{Structure
+|PDB= 1bc7 |SIZE=350|CAPTION= <scene name='initialview01'>1bc7</scene>, resolution 2.01&Aring;
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE= SAP-1 RESIDUES 1-93 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+}}
+'''SERUM RESPONSE FACTOR ACCESSORY PROTEIN 1A (SAP-1)/DNA COMPLEX'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-BC7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BC7 OCA].
+BC7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BC7 OCA].
 ==Reference==
-Structures of SAP-1 bound to DNA targets from the E74 and c-fos promoters: insights into DNA sequence discrimination by Ets proteins., Mo Y, Vaessen B, Johnston K, Marmorstein R, Mol Cell. 1998 Aug;2(2):201-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9734357 9734357]
+Structures of SAP-1 bound to DNA targets from the E74 and c-fos promoters: insights into DNA sequence discrimination by Ets proteins., Mo Y, Vaessen B, Johnston K, Marmorstein R, Mol Cell. 1998 Aug;2(2):201-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9734357 9734357]
 [[Category: Homo sapiens]]
 [[Category: Single protein]]
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 [[Category: winged helix-turn-helix]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:53:44 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:09:02 2008''