1b92: Difference between revisions
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'''MOBILITY OF AN HIV-1 INTEGRASE ACTIVE SITE LOOP IS CORRELATED WITH CATALYTIC ACTIVITY''' | {{Structure | ||
|PDB= 1b92 |SIZE=350|CAPTION= <scene name='initialview01'>1b92</scene>, resolution 2.02Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene> and <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/RNA-directed_DNA_polymerase RNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49] | |||
|GENE= | |||
}} | |||
'''MOBILITY OF AN HIV-1 INTEGRASE ACTIVE SITE LOOP IS CORRELATED WITH CATALYTIC ACTIVITY''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1B92 is a [ | 1B92 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_type_1_(isolate_12) Human immunodeficiency virus type 1 (isolate 12)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B92 OCA]. | ||
==Reference== | ==Reference== | ||
The mobility of an HIV-1 integrase active site loop is correlated with catalytic activity., Greenwald J, Le V, Butler SL, Bushman FD, Choe S, Biochemistry. 1999 Jul 13;38(28):8892-8. PMID:[http:// | The mobility of an HIV-1 integrase active site loop is correlated with catalytic activity., Greenwald J, Le V, Butler SL, Bushman FD, Choe S, Biochemistry. 1999 Jul 13;38(28):8892-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10413462 10413462] | ||
[[Category: Human immunodeficiency virus type 1 (isolate 12)]] | [[Category: Human immunodeficiency virus type 1 (isolate 12)]] | ||
[[Category: RNA-directed DNA polymerase]] | [[Category: RNA-directed DNA polymerase]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:07:52 2008'' | ||
Revision as of 11:07, 20 March 2008
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| , resolution 2.02Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Activity: | RNA-directed DNA polymerase, with EC number 2.7.7.49 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
MOBILITY OF AN HIV-1 INTEGRASE ACTIVE SITE LOOP IS CORRELATED WITH CATALYTIC ACTIVITY
OverviewOverview
Replication of HIV-1 requires the covalent integration of the viral cDNA into the host chromosomal DNA directed by the virus-encoded integrase protein. Here we explore the importance of a protein surface loop near the integrase active site using protein engineering and X-ray crystallography. We have redetermined the structure of the integrase catalytic domain (residues 50-212) using an independent phase set at 1.7 A resolution. The structure extends helix alpha4 on its N-terminal side (residues 149-154), thus defining the position of the three conserved active site residues. Evident in this and in previous structures is a conformationally flexible loop composed of residues 141-148. To probe the role of flexibility in this loop, we replaced Gly 140 and Gly 149, residues that appear to act as conformational hinges, with Ala residues. X-ray structures of the catalytic domain mutants G149A and G140A/G149A show further rigidity of alpha4 and the adjoining loop. Activity assays in vitro revealed that these mutants are impaired in catalysis. The DNA binding affinity, however, is minimally affected by these mutants as assayed by UV cross-linking. We propose that the conformational flexibility of this active site loop is important for a postbinding catalytic step.
About this StructureAbout this Structure
1B92 is a Single protein structure of sequence from Human immunodeficiency virus type 1 (isolate 12). Full crystallographic information is available from OCA.
ReferenceReference
The mobility of an HIV-1 integrase active site loop is correlated with catalytic activity., Greenwald J, Le V, Butler SL, Bushman FD, Choe S, Biochemistry. 1999 Jul 13;38(28):8892-8. PMID:10413462
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