1b63: Difference between revisions

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Revision as of 11:06, 20 March 2008

File:1b63.gif

, resolution 1.9Å

Ligands:

, and

Gene:

MUTL (Escherichia coli)

Coordinates:

save as pdb, mmCIF, xml

MUTL COMPLEXED WITH ADPNP

OverviewOverview

The MutL DNA mismatch repair protein has recently been shown to be an ATPase and to belong to an emerging ATPase superfamily that includes DNA topoisomerase II and Hsp90. We report here the crystal structures of a 40 kDa ATPase fragment of E. coli MutL (LN40) complexed with a substrate analog, ADPnP, and with product ADP. More than 60 residues that are disordered in the apoprotein structure become ordered and contribute to both ADPnP binding and dimerization of LN40. Hydrolysis of ATP, signified by subsequent release of the gamma-phosphate, releases two key loops and leads to dissociation of the LN40 dimer. Dimerization of the LN40 region is required for and is the rate-limiting step in ATP hydrolysis by MutL. The ATPase activity of MutL is stimulated by DNA and likely acts as a switch to coordinate DNA mismatch repair.

About this StructureAbout this Structure

1B63 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Transformation of MutL by ATP binding and hydrolysis: a switch in DNA mismatch repair., Ban C, Junop M, Yang W, Cell. 1999 Apr 2;97(1):85-97. PMID:10199405

Page seeded by OCA on Thu Mar 20 10:06:45 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1b63.gif|left|200px]]<br /><applet load="1b63" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1b63.gif|left|200px]]
-caption="1b63, resolution 1.9&Aring;" />
-'''MUTL COMPLEXED WITH ADPNP'''<br />
+ {{Structure
+|PDB= 1b63 |SIZE=350|CAPTION= <scene name='initialview01'>1b63</scene>, resolution 1.9&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene> and <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER'>ANP</scene>
+|ACTIVITY=
+|GENE= MUTL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+}}
+'''MUTL COMPLEXED WITH ADPNP'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-B63 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=EDO:'>EDO</scene> and <scene name='pdbligand=ANP:'>ANP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B63 OCA].
+B63 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B63 OCA].
 ==Reference==
-Transformation of MutL by ATP binding and hydrolysis: a switch in DNA mismatch repair., Ban C, Junop M, Yang W, Cell. 1999 Apr 2;97(1):85-97. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10199405 10199405]
+Transformation of MutL by ATP binding and hydrolysis: a switch in DNA mismatch repair., Ban C, Junop M, Yang W, Cell. 1999 Apr 2;97(1):85-97. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10199405 10199405]
 [[Category: Escherichia coli]]
 [[Category: Single protein]]
@@ Line 20: / Line 29: @@
 [[Category: dna mismatch repair]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:51:52 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:06:45 2008''