2c7q: Difference between revisions
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Revision as of 17:56, 30 October 2007
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HHAI DNA METHYLTRANSFERASE COMPLEX WITH OLIGONUCLEOTIDE CONTAINING 2-AMINOPURINE OUTSIDE THE RECOGNITION SEQUENCE (PAIRED WITH G) AND SAH
OverviewOverview
DNA base flipping is an important mechanism in molecular enzymology, but, its study is limited by the lack of an accessible and reliable diagnostic, technique. A series of crystalline complexes of a DNA methyltransferase, M.HhaI, and its cognate DNA, in which a fluorescent nucleobase analogue, 2-aminopurine (AP), occupies defined positions with respect the target, flipped base, have been prepared and their structures determined at higher, than 2 A resolution. From time-resolved fluorescence measurements of these, single crystals, we have established that the fluorescence decay function, of AP shows a pronounced, characteristic response to base flipping: the, loss of the very short (approximately 100 ps) decay component and the, large increase in the amplitude of the long (approximately 10 ... [(full description)]
About this StructureAbout this Structure
2C7Q is a [Protein complex] structure of sequences from [Haemophilus haemolyticus] with SO4, SAH and CIT as [ligands]. Active as [DNA (cytosine-5-)-methyltransferase], with EC number [2.1.1.37]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
Time-resolved fluorescence of 2-aminopurine as a probe of base flipping in M.HhaI-DNA complexes., Neely RK, Daujotyte D, Grazulis S, Magennis SW, Dryden DT, Klimasauskas S, Jones AC, Nucleic Acids Res. 2005 Dec 9;33(22):6953-60. Print 2005. PMID:16340006
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