1azt: Difference between revisions

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Revision as of 11:04, 20 March 2008

File:1azt.jpg

, resolution 2.3Å

Ligands:

, and

Gene:

GNAS (Bos taurus)

Coordinates:

save as pdb, mmCIF, xml

GS-ALPHA COMPLEXED WITH GTP-GAMMA-S

OverviewOverview

The crystal structure of Gsalpha, the heterotrimeric G protein alpha subunit that stimulates adenylyl cyclase, was determined at 2.5 A in a complex with guanosine 5'-O-(3-thiotriphosphate) (GTPgammaS). Gsalpha is the prototypic member of a family of GTP-binding proteins that regulate the activities of effectors in a hormone-dependent manner. Comparison of the structure of Gsalpha.GTPgammaS with that of Gialpha.GTPgammaS suggests that their effector specificity is primarily dictated by the shape of the binding surface formed by the switch II helix and the alpha3-beta5 loop, despite the high sequence homology of these elements. In contrast, sequence divergence explains the inability of regulators of G protein signaling to stimulate the GTPase activity of Gsalpha. The betagamma binding surface of Gsalpha is largely conserved in sequence and structure to that of Gialpha, whereas differences in the surface formed by the carboxyl-terminal helix and the alpha4-beta6 loop may mediate receptor specificity.

About this StructureAbout this Structure

1AZT is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the adenylyl cyclase activator Gsalpha., Sunahara RK, Tesmer JJ, Gilman AG, Sprang SR, Science. 1997 Dec 12;278(5345):1943-7. PMID:9395396

Page seeded by OCA on Thu Mar 20 10:04:23 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1azt.jpg|left|200px]]<br /><applet load="1azt" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1azt.jpg|left|200px]]
-caption="1azt, resolution 2.3&Aring;" />
-'''GS-ALPHA COMPLEXED WITH GTP-GAMMA-S'''<br />
+ {{Structure
+|PDB= 1azt |SIZE=350|CAPTION= <scene name='initialview01'>1azt</scene>, resolution 2.3&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> and <scene name='pdbligand=GSP:5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE'>GSP</scene>
+|ACTIVITY=
+|GENE= GNAS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 Bos taurus])
+}}
+'''GS-ALPHA COMPLEXED WITH GTP-GAMMA-S'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-AZT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=GSP:'>GSP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AZT OCA].
+AZT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AZT OCA].
 ==Reference==
-Crystal structure of the adenylyl cyclase activator Gsalpha., Sunahara RK, Tesmer JJ, Gilman AG, Sprang SR, Science. 1997 Dec 12;278(5345):1943-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9395396 9395396]
+Crystal structure of the adenylyl cyclase activator Gsalpha., Sunahara RK, Tesmer JJ, Gilman AG, Sprang SR, Science. 1997 Dec 12;278(5345):1943-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9395396 9395396]
 [[Category: Bos taurus]]
 [[Category: Single protein]]
@@ Line 22: / Line 31: @@
 [[Category: signal transducing protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:50:00 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:04:23 2008''