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HUMAN PLATELET PROFILIN COMPLEXED WITH THE L-PRO10 PEPTIDE

OverviewOverview

Profilin, a ubiquitous low molecular weight (13,000-15,000 M(r)) actin binding protein, regulates the formation of F-actin structures in vivo, and is localized to specific cellular regions through interaction with proline-rich sequences. Here we report the 2.2 A X-ray structure of the complex between human platelet profilin (HPP) and a decamer of L-proline (L-Pro10). The L-Pro10 peptide adopts a left-handed type II poly-L-proline helix (PPII) and binds to a highly conserved patch of aromatic amino acids on the surface of profilin. The peptide and actin binding sites reside on orthogonal surfaces, and L-Pro10 binding does not result in a conformational rearrangement of HPP. This structure suggests a mechanism for the localization of profilin and its actin-related activities to sites of actin filament assembly in vivo.

About this StructureAbout this Structure

1AWI is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structure of the profilin-poly-L-proline complex involved in morphogenesis and cytoskeletal regulation., Mahoney NM, Janmey PA, Almo SC, Nat Struct Biol. 1997 Nov;4(11):953-60. PMID:9360613

Page seeded by OCA on Thu Mar 20 10:03:10 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1awi.gif|left|200px]]<br /><applet load="1awi" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1awi.gif|left|200px]]
-caption="1awi, resolution 2.2&Aring;" />
-'''HUMAN PLATELET PROFILIN COMPLEXED WITH THE L-PRO10 PEPTIDE'''<br />
+ {{Structure
+|PDB= 1awi |SIZE=350|CAPTION= <scene name='initialview01'>1awi</scene>, resolution 2.2&Aring;
+|SITE= <scene name='pdbsite=PPA:Poly-L-PRO+Binding+Site'>PPA</scene> and <scene name='pdbsite=PPB:Poly-L-PRO+Binding+Site'>PPB</scene>
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''HUMAN PLATELET PROFILIN COMPLEXED WITH THE L-PRO10 PEPTIDE'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-AWI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Known structural/functional Sites: <scene name='pdbsite=PPA:Poly-L-PRO+Binding+Site'>PPA</scene> and <scene name='pdbsite=PPB:Poly-L-PRO+Binding+Site'>PPB</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AWI OCA].
+AWI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AWI OCA].
 ==Reference==
-Structure of the profilin-poly-L-proline complex involved in morphogenesis and cytoskeletal regulation., Mahoney NM, Janmey PA, Almo SC, Nat Struct Biol. 1997 Nov;4(11):953-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9360613 9360613]
+Structure of the profilin-poly-L-proline complex involved in morphogenesis and cytoskeletal regulation., Mahoney NM, Janmey PA, Almo SC, Nat Struct Biol. 1997 Nov;4(11):953-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9360613 9360613]
 [[Category: Homo sapiens]]
 [[Category: Single protein]]
@@ Line 20: / Line 29: @@
 [[Category: profilin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:48:58 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:03:10 2008''