1avq: Difference between revisions

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Revision as of 11:02, 20 March 2008

File:1avq.gif

, resolution 2.4Å

Ligands:

and

Activity:

Exodeoxyribonuclease (lambda-induced), with EC number 3.1.11.3

Coordinates:

save as pdb, mmCIF, xml

TOROIDAL STRUCTURE OF LAMBDA EXONUCLEASE DETERMINED AT 2.4 ANGSTROMS

OverviewOverview

Structure determination at 2.4 angstrom resolution shows that lambda-exonuclease consists of three subunits that form a toroid. The central channel is funnel shaped, tapering from an inner diameter of about 30 angstroms at the wider end to 15 angstroms at the narrow end. This is adequate to accommodate the DNA substrate and thus provides a structural basis for the ability of the enzyme to sequentially hydrolyze thousands of nucleotides in a highly processive manner. The results also suggest the locations of the active sites and the constraints that limit cleavage to a single strand.

About this StructureAbout this Structure

1AVQ is a Single protein structure of sequence from Enterobacteria phage lambda. Full crystallographic information is available from OCA.

ReferenceReference

Toroidal structure of lambda-exonuclease., Kovall R, Matthews BW, Science. 1997 Sep 19;277(5333):1824-7. PMID:9295273

Page seeded by OCA on Thu Mar 20 10:02:50 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1avq.gif|left|200px]]<br /><applet load="1avq" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1avq.gif|left|200px]]
-caption="1avq, resolution 2.4&Aring;" />
-'''TOROIDAL STRUCTURE OF LAMBDA EXONUCLEASE DETERMINED AT 2.4 ANGSTROMS'''<br />
+ {{Structure
+|PDB= 1avq |SIZE=350|CAPTION= <scene name='initialview01'>1avq</scene>, resolution 2.4&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> and <scene name='pdbligand=ACT:ACETATE ION'>ACT</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/Exodeoxyribonuclease_(lambda-induced) Exodeoxyribonuclease (lambda-induced)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.11.3 3.1.11.3]
+|GENE=
+}}
+'''TOROIDAL STRUCTURE OF LAMBDA EXONUCLEASE DETERMINED AT 2.4 ANGSTROMS'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-AVQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda] with <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=ACT:'>ACT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Exodeoxyribonuclease_(lambda-induced) Exodeoxyribonuclease (lambda-induced)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.11.3 3.1.11.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AVQ OCA].
+AVQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AVQ OCA].
 ==Reference==
-Toroidal structure of lambda-exonuclease., Kovall R, Matthews BW, Science. 1997 Sep 19;277(5333):1824-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9295273 9295273]
+Toroidal structure of lambda-exonuclease., Kovall R, Matthews BW, Science. 1997 Sep 19;277(5333):1824-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9295273 9295273]
 [[Category: Enterobacteria phage lambda]]
 [[Category: Exodeoxyribonuclease (lambda-induced)]]
@@ Line 22: / Line 31: @@
 [[Category: dna recombination and repair]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:48:46 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:02:50 2008''