1ahe: Difference between revisions

← Older edit Newer edit →

Revision as of 10:57, 20 March 2008

File:1ahe.gif

, resolution 2.3Å

Ligands:

and

Activity:

Aspartate transaminase, with EC number 2.6.1.1

Coordinates:

save as pdb, mmCIF, xml

ASPARTATE AMINOTRANSFERASE HEXAMUTANT

OverviewOverview

Mutation of six residues of Escherichia coli aspartate aminotransferase results in substantial acquisition of the transamination properties of tyrosine amino-transferase without loss of aspartate transaminase activity. X-ray crystallographic analysis of key inhibitor complexes of the hexamutant reveals the structural basis for this substrate selectivity. It appears that tyrosine aminotransferase achieves nearly equal affinities for a wide range of amino acids by an unusual conformational switch. An active-site arginine residue either shifts its position to electrostatically interact with charged substrates or moves aside to allow access of aromatic ligands.

About this StructureAbout this Structure

1AHE is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Alternating arginine-modulated substrate specificity in an engineered tyrosine aminotransferase., Malashkevich VN, Onuffer JJ, Kirsch JF, Jansonius JN, Nat Struct Biol. 1995 Jul;2(7):548-53. PMID:7664122

Page seeded by OCA on Thu Mar 20 09:57:33 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1ahe.gif|left|200px]]<br /><applet load="1ahe" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ahe.gif|left|200px]]
-caption="1ahe, resolution 2.3&Aring;" />
-'''ASPARTATE AMINOTRANSFERASE HEXAMUTANT'''<br />
+ {{Structure
+|PDB= 1ahe |SIZE=350|CAPTION= <scene name='initialview01'>1ahe</scene>, resolution 2.3&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=PLP:PYRIDOXAL-5'-PHOSPHATE'>PLP</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/Aspartate_transaminase Aspartate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 2.6.1.1]
+|GENE=
+}}
+'''ASPARTATE AMINOTRANSFERASE HEXAMUTANT'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-AHE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=PLP:'>PLP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Aspartate_transaminase Aspartate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 2.6.1.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AHE OCA].
+AHE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AHE OCA].
 ==Reference==
-Alternating arginine-modulated substrate specificity in an engineered tyrosine aminotransferase., Malashkevich VN, Onuffer JJ, Kirsch JF, Jansonius JN, Nat Struct Biol. 1995 Jul;2(7):548-53. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7664122 7664122]
+Alternating arginine-modulated substrate specificity in an engineered tyrosine aminotransferase., Malashkevich VN, Onuffer JJ, Kirsch JF, Jansonius JN, Nat Struct Biol. 1995 Jul;2(7):548-53. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7664122 7664122]
 [[Category: Aspartate transaminase]]
 [[Category: Escherichia coli]]
@@ Line 20: / Line 29: @@
 [[Category: transferase (aminotransferase)]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:44:30 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:57:33 2008''