1agr: Difference between revisions

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Revision as of 10:57, 20 March 2008

File:1agr.gif

, resolution 2.8Å

Ligands:

, , and

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COMPLEX OF ALF4-ACTIVATED GI-ALPHA-1 WITH RGS4

OverviewOverview

RGS proteins are GTPase activators for heterotrimeric G proteins. We report here the 2.8 A resolution crystal structure of the RGS protein RGS4 complexed with G(i alpha1)-Mg2+-GDP-AlF4 . Only the core domain of RGS4 is visible in the crystal. The core domain binds to the three switch regions of G(i alpha1), but does not contribute catalytic residues that directly interact with either GDP or AlF4-. Therefore, RGS4 appears to catalyze rapid hydrolysis of GTP primarily by stabilizing the switch regions of G(i alpha1), although the conserved Asn-128 from RGS4 could also play a catalytic role by interacting with the hydrolytic water molecule or the side chain of Gln-204. The binding site for RGS4 on G(i alpha1) is also consistent with the activity of RGS proteins as antagonists of G(alpha) effectors.

About this StructureAbout this Structure

1AGR is a Protein complex structure of sequences from Rattus norvegicus. Full crystallographic information is available from OCA.

ReferenceReference

Structure of RGS4 bound to AlF4--activated G(i alpha1): stabilization of the transition state for GTP hydrolysis., Tesmer JJ, Berman DM, Gilman AG, Sprang SR, Cell. 1997 Apr 18;89(2):251-61. PMID:9108480

Page seeded by OCA on Thu Mar 20 09:57:18 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1agr.gif|left|200px]]<br /><applet load="1agr" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1agr.gif|left|200px]]
-caption="1agr, resolution 2.8&Aring;" />
-'''COMPLEX OF ALF4-ACTIVATED GI-ALPHA-1 WITH RGS4'''<br />
+ {{Structure
+|PDB= 1agr |SIZE=350|CAPTION= <scene name='initialview01'>1agr</scene>, resolution 2.8&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=GDP:GUANOSINE-5'-DIPHOSPHATE'>GDP</scene> and <scene name='pdbligand=CIT:CITRIC ACID'>CIT</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''COMPLEX OF ALF4-ACTIVATED GI-ALPHA-1 WITH RGS4'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-AGR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=ALF:'>ALF</scene>, <scene name='pdbligand=GDP:'>GDP</scene> and <scene name='pdbligand=CIT:'>CIT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AGR OCA].
+AGR is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AGR OCA].
 ==Reference==
-Structure of RGS4 bound to AlF4--activated G(i alpha1): stabilization of the transition state for GTP hydrolysis., Tesmer JJ, Berman DM, Gilman AG, Sprang SR, Cell. 1997 Apr 18;89(2):251-61. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9108480 9108480]
+Structure of RGS4 bound to AlF4--activated G(i alpha1): stabilization of the transition state for GTP hydrolysis., Tesmer JJ, Berman DM, Gilman AG, Sprang SR, Cell. 1997 Apr 18;89(2):251-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9108480 9108480]
 [[Category: Protein complex]]
 [[Category: Rattus norvegicus]]
@@ Line 27: / Line 36: @@
 [[Category: signal transduction]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:44:23 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:57:18 2008''