1ae6: Difference between revisions

Revision as of 10:56, 20 March 2008

, resolution 3.00Å

Coordinates:

IGG-FAB FRAGMENT OF MOUSE MONOCLONAL ANTIBODY CTM01

OverviewOverview

The crystal structures of two pairs of Fab fragments have been determined. The pairs comprise both a murine and an engineered human form, each derived from the antitumor antibodies A5B7 and CTM01. Although antigen specificity is maintained within the pairs, antigen affinity varies. A comparison of the hypervariable loops for each pair of antibodies shows their structure has been well maintained in grafting, supporting the canonical loop model. Detailed structural analysis of the binding sites and domain arrangements for these antibodies suggests the differences in antigen affinity observed are likely to be due to inherent flexibility of the hypervariable loops and movements at the VL:VH domain interface. The four structures provide the first opportunity to study in detail the effects of protein engineering on specific antibodies.

About this StructureAbout this Structure

1AE6 is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

VL:VH domain rotations in engineered antibodies: crystal structures of the Fab fragments from two murine antitumor antibodies and their engineered human constructs., Banfield MJ, King DJ, Mountain A, Brady RL, Proteins. 1997 Oct;29(2):161-71. PMID:9329081

Page seeded by OCA on Thu Mar 20 09:56:16 2008

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OCA

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-[[Image:1ae6.jpg|left|200px]]<br /><applet load="1ae6" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ae6.jpg|left|200px]]
-caption="1ae6, resolution 3.00&Aring;" />
-'''IGG-FAB FRAGMENT OF MOUSE MONOCLONAL ANTIBODY CTM01'''<br />
+ {{Structure
+|PDB= 1ae6 |SIZE=350|CAPTION= <scene name='initialview01'>1ae6</scene>, resolution 3.00&Aring;
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''IGG-FAB FRAGMENT OF MOUSE MONOCLONAL ANTIBODY CTM01'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-AE6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE6 OCA].
+AE6 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE6 OCA].
 ==Reference==
-VL:VH domain rotations in engineered antibodies: crystal structures of the Fab fragments from two murine antitumor antibodies and their engineered human constructs., Banfield MJ, King DJ, Mountain A, Brady RL, Proteins. 1997 Oct;29(2):161-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9329081 9329081]
+VL:VH domain rotations in engineered antibodies: crystal structures of the Fab fragments from two murine antitumor antibodies and their engineered human constructs., Banfield MJ, King DJ, Mountain A, Brady RL, Proteins. 1997 Oct;29(2):161-71. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9329081 9329081]
 [[Category: Mus musculus]]
 [[Category: Protein complex]]
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 [[Category: immunoglobulin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:43:29 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:56:16 2008''