1ad9: Difference between revisions

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IGG-FAB FRAGMENT OF ENGINEERED HUMAN MONOCLONAL ANTIBODY CTM01

OverviewOverview

The crystal structures of two pairs of Fab fragments have been determined. The pairs comprise both a murine and an engineered human form, each derived from the antitumor antibodies A5B7 and CTM01. Although antigen specificity is maintained within the pairs, antigen affinity varies. A comparison of the hypervariable loops for each pair of antibodies shows their structure has been well maintained in grafting, supporting the canonical loop model. Detailed structural analysis of the binding sites and domain arrangements for these antibodies suggests the differences in antigen affinity observed are likely to be due to inherent flexibility of the hypervariable loops and movements at the VL:VH domain interface. The four structures provide the first opportunity to study in detail the effects of protein engineering on specific antibodies.

About this StructureAbout this Structure

1AD9 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

VL:VH domain rotations in engineered antibodies: crystal structures of the Fab fragments from two murine antitumor antibodies and their engineered human constructs., Banfield MJ, King DJ, Mountain A, Brady RL, Proteins. 1997 Oct;29(2):161-71. PMID:9329081

Page seeded by OCA on Thu Mar 20 09:55:58 2008

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OCA

@@ Line 1: / Line 1: @@
-[[Image:1ad9.jpg|left|200px]]<br /><applet load="1ad9" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ad9.jpg|left|200px]]
-caption="1ad9, resolution 2.80&Aring;" />
-'''IGG-FAB FRAGMENT OF ENGINEERED HUMAN MONOCLONAL ANTIBODY CTM01'''<br />
+ {{Structure
+|PDB= 1ad9 |SIZE=350|CAPTION= <scene name='initialview01'>1ad9</scene>, resolution 2.80&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''IGG-FAB FRAGMENT OF ENGINEERED HUMAN MONOCLONAL ANTIBODY CTM01'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-AD9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AD9 OCA].
+AD9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AD9 OCA].
 ==Reference==
-VL:VH domain rotations in engineered antibodies: crystal structures of the Fab fragments from two murine antitumor antibodies and their engineered human constructs., Banfield MJ, King DJ, Mountain A, Brady RL, Proteins. 1997 Oct;29(2):161-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9329081 9329081]
+VL:VH domain rotations in engineered antibodies: crystal structures of the Fab fragments from two murine antitumor antibodies and their engineered human constructs., Banfield MJ, King DJ, Mountain A, Brady RL, Proteins. 1997 Oct;29(2):161-71. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9329081 9329081]
 [[Category: Homo sapiens]]
 [[Category: Single protein]]
@@ Line 19: / Line 28: @@
 [[Category: immunoglobulin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:43:16 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:55:58 2008''