1abs: Difference between revisions

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Revision as of 10:55, 20 March 2008

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PHOTOLYSED CARBONMONOXY-MYOGLOBIN AT 20 K

OverviewOverview

Myoglobin is a globular haem protein that reversibly binds ligands such as O2 and CO. Single photons of visible light can break the covalent bond between CO and the haem iron in carbon-monoxy-myoglobin (MbCO) and thus form an unstable intermediate, Mb*CO, with the CO inside the protein. The ensuing rebinding process has been extensively studied as a model for the interplay of dynamics, structure and function in protein reactions. We have used X-ray crystallography at liquid-helium temperatures to determine the structure of Mb*CO to a resolution of 1.5 A. The photodissociated CO lies on top of the haem pyrrole ring C. Comparison with the CO-bound and unligated myoglobin structures reveals that on photodissociation of the CO, the haem 'domes', the iron moves partially out of the haem plane, the iron-proximal histidine bonds is compressed, the F helix is strained and the distal histidine swings towards the outside of the ligand-binding pocket.

About this StructureAbout this Structure

1ABS is a Single protein structure of sequence from Physeter catodon. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of photolysed carbonmonoxy-myoglobin., Schlichting I, Berendzen J, Phillips GN Jr, Sweet RM, Nature. 1994 Oct 27;371(6500):808-12. PMID:7935843

Page seeded by OCA on Thu Mar 20 09:55:30 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1abs.jpg|left|200px]]<br /><applet load="1abs" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1abs.jpg|left|200px]]
-caption="1abs, resolution 1.5&Aring;" />
-'''PHOTOLYSED CARBONMONOXY-MYOGLOBIN AT 20 K'''<br />
+ {{Structure
+|PDB= 1abs |SIZE=350|CAPTION= <scene name='initialview01'>1abs</scene>, resolution 1.5&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene> and <scene name='pdbligand=CMO:CARBON MONOXIDE'>CMO</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''PHOTOLYSED CARBONMONOXY-MYOGLOBIN AT 20 K'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-ABS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Physeter_catodon Physeter catodon] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=HEM:'>HEM</scene> and <scene name='pdbligand=CMO:'>CMO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ABS OCA].
+ABS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Physeter_catodon Physeter catodon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ABS OCA].
 ==Reference==
-Crystal structure of photolysed carbonmonoxy-myoglobin., Schlichting I, Berendzen J, Phillips GN Jr, Sweet RM, Nature. 1994 Oct 27;371(6500):808-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7935843 7935843]
+Crystal structure of photolysed carbonmonoxy-myoglobin., Schlichting I, Berendzen J, Phillips GN Jr, Sweet RM, Nature. 1994 Oct 27;371(6500):808-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7935843 7935843]
 [[Category: Physeter catodon]]
 [[Category: Single protein]]
@@ Line 24: / Line 33: @@
 [[Category: respiratory protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:42:57 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:55:30 2008''