3wae: Difference between revisions
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''' | ==X-ray structure of Fe(III)-bicarbonates-ttfbpa, a ferric ion-binding protein from thermus thermophilus HB8== | ||
<StructureSection load='3wae' size='340' side='right' caption='[[3wae]], [[Resolution|resolution]] 1.70Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3wae]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WAE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WAE FirstGlance]. <br> | |||
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BCT:BICARBONATE+ION'>BCT</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene><br> | |||
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3waf|3waf]]</td></tr> | |||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wae FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wae OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wae RCSB], [http://www.ebi.ac.uk/pdbsum/3wae PDBsum]</span></td></tr> | |||
<table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Crystal structures of FbpA, the periplasmic ferric ion-binding protein of an iron-uptake ABC transporter, from Thermus thermophilus HB8 (TtFbpA) have been solved in apo and ferric ion-bound forms at 1.8 and 1.7 A resolution, respectively. The latter crystal structure shows that the bound ferric ion forms a novel six-coordinated complex with three tyrosine side chains, two bicarbonates and a water molecule in the metal-binding site. The results of gel-filtration chromatography and dynamic light scattering show that TtFbpA exists as a monomer in solution regardless of ferric ion binding and that TtFbpA adopts a more compact conformation in the ferric ion-bound state than in the apo state in solution. | |||
A novel mode of ferric ion coordination by the periplasmic ferric ion-binding subunit FbpA of an ABC-type iron transporter from Thermus thermophilus HB8.,Wang S, Ogata M, Horita S, Ohtsuka J, Nagata K, Tanokura M Acta Crystallogr D Biol Crystallogr. 2014 Jan;70(Pt 1):196-202. doi:, 10.1107/S1399004713026333. Epub 2013 Dec 31. PMID:24419392<ref>PMID:24419392</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Horita, S.]] | |||
[[Category: Nagata, K.]] | |||
[[Category: Ogata, M.]] | |||
[[Category: Ohtsuka, J.]] | |||
[[Category: Tanokura, M.]] | |||
[[Category: Wang, S.]] | |||
[[Category: A ferric binding protein]] | |||
[[Category: Ferric ion]] | |||
[[Category: Metal binding protein]] | |||
Revision as of 11:58, 21 May 2014
X-ray structure of Fe(III)-bicarbonates-ttfbpa, a ferric ion-binding protein from thermus thermophilus HB8X-ray structure of Fe(III)-bicarbonates-ttfbpa, a ferric ion-binding protein from thermus thermophilus HB8
Structural highlights
Publication Abstract from PubMedCrystal structures of FbpA, the periplasmic ferric ion-binding protein of an iron-uptake ABC transporter, from Thermus thermophilus HB8 (TtFbpA) have been solved in apo and ferric ion-bound forms at 1.8 and 1.7 A resolution, respectively. The latter crystal structure shows that the bound ferric ion forms a novel six-coordinated complex with three tyrosine side chains, two bicarbonates and a water molecule in the metal-binding site. The results of gel-filtration chromatography and dynamic light scattering show that TtFbpA exists as a monomer in solution regardless of ferric ion binding and that TtFbpA adopts a more compact conformation in the ferric ion-bound state than in the apo state in solution. A novel mode of ferric ion coordination by the periplasmic ferric ion-binding subunit FbpA of an ABC-type iron transporter from Thermus thermophilus HB8.,Wang S, Ogata M, Horita S, Ohtsuka J, Nagata K, Tanokura M Acta Crystallogr D Biol Crystallogr. 2014 Jan;70(Pt 1):196-202. doi:, 10.1107/S1399004713026333. Epub 2013 Dec 31. PMID:24419392[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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