1a8r: Difference between revisions
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'''GTP CYCLOHYDROLASE I (H112S MUTANT) IN COMPLEX WITH GTP''' | {{Structure | ||
|PDB= 1a8r |SIZE=350|CAPTION= <scene name='initialview01'>1a8r</scene>, resolution 2.1Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=GTP:GUANOSINE-5'-TRIPHOSPHATE'>GTP</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/GTP_cyclohydrolase_I GTP cyclohydrolase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.4.16 3.5.4.16] | |||
|GENE= | |||
}} | |||
'''GTP CYCLOHYDROLASE I (H112S MUTANT) IN COMPLEX WITH GTP''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1A8R is a [ | 1A8R is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A8R OCA]. | ||
==Reference== | ==Reference== | ||
Biosynthesis of pteridines. Reaction mechanism of GTP cyclohydrolase I., Rebelo J, Auerbach G, Bader G, Bracher A, Nar H, Hosl C, Schramek N, Kaiser J, Bacher A, Huber R, Fischer M, J Mol Biol. 2003 Feb 14;326(2):503-16. PMID:[http:// | Biosynthesis of pteridines. Reaction mechanism of GTP cyclohydrolase I., Rebelo J, Auerbach G, Bader G, Bracher A, Nar H, Hosl C, Schramek N, Kaiser J, Bacher A, Huber R, Fischer M, J Mol Biol. 2003 Feb 14;326(2):503-16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12559918 12559918] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: GTP cyclohydrolase I]] | [[Category: GTP cyclohydrolase I]] | ||
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[[Category: purine hydrolysis]] | [[Category: purine hydrolysis]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:54:31 2008'' | ||
Revision as of 10:54, 20 March 2008
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| , resolution 2.1Å | |||||||
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| Ligands: | |||||||
| Activity: | GTP cyclohydrolase I, with EC number 3.5.4.16 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
GTP CYCLOHYDROLASE I (H112S MUTANT) IN COMPLEX WITH GTP
OverviewOverview
GTP cyclohydrolase I catalyses the hydrolytic release of formate from GTP followed by cyclization to dihydroneopterin triphosphate. The enzymes from bacteria and animals are homodecamers containing one zinc ion per subunit. Replacement of Cys110, Cys181, His112 or His113 of the enzyme from Escherichia coli by serine affords catalytically inactive mutant proteins with reduced capacity to bind zinc. These mutant proteins are unable to convert GTP or the committed reaction intermediate, 2-amino-5-formylamino-6-(beta-ribosylamino)-4(3H)-pyrimidinone 5'-triphosphate, to dihydroneopterin triphosphate. The crystal structures of GTP complexes of the His113Ser, His112Ser and Cys181Ser mutant proteins determined at resolutions of 2.5A, 2.8A and 3.2A, respectively, revealed the conformation of substrate GTP in the active site cavity. The carboxylic group of the highly conserved residue Glu152 anchors the substrate GTP, by hydrogen bonding to N-3 and to the position 2 amino group. Several basic amino acid residues interact with the triphosphate moiety of the substrate. The structure of the His112Ser mutant in complex with an undefined mixture of nucleotides determined at a resolution of 2.1A afforded additional details of the peptide folding. Comparison between the wild-type and mutant enzyme structures indicates that the catalytically active zinc ion is directly coordinated to Cys110, Cys181 and His113. Moreover, the zinc ion is complexed to a water molecule, which is in close hydrogen bond contact to His112. In close analogy to zinc proteases, the zinc-coordinated water molecule is suggested to attack C-8 of the substrate affording a zinc-bound 8R hydrate of GTP. Opening of the hydrated imidazole ring affords a formamide derivative, which remains coordinated to zinc. The subsequent hydrolysis of the formamide motif has an absolute requirement for zinc ion catalysis. The hydrolysis of the formamide bond shows close mechanistic similarity with peptide hydrolysis by zinc proteases.
About this StructureAbout this Structure
1A8R is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Biosynthesis of pteridines. Reaction mechanism of GTP cyclohydrolase I., Rebelo J, Auerbach G, Bader G, Bracher A, Nar H, Hosl C, Schramek N, Kaiser J, Bacher A, Huber R, Fischer M, J Mol Biol. 2003 Feb 14;326(2):503-16. PMID:12559918
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