1a6i: Difference between revisions

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File:1a6i.gif

, resolution 2.4Å

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TET REPRESSOR, CLASS D VARIANT

OverviewOverview

The X-ray crystal structure analysis of inducer-free Tet repressor, TetR, at 2.4 A resolution identifies one of two openings of the tunnel-like binding site as the entrance for the inducer tetracycline-Mg2+, [Mg Tc]+. Recognition and binding of the inducer unleashes conformational changes leading to the induced state of TetR. In the first step, the C-terminal turn of alpha-helix 6 unwinds, thereby altering the orientation of alpha-helix 4. This different orientation of alpha-helix 4 is stabilized by a series of hydrogen bonds mediated through a chain of eight water molecules. The alpha-helix 4 connects the DNA-binding domain (alpha-helices 1 to 3) to the rigid TetR core, and thus regulates gene expression through its respective orientations.

About this StructureAbout this Structure

1A6I is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Conformational changes of the Tet repressor induced by tetracycline trapping., Orth P, Cordes F, Schnappinger D, Hillen W, Saenger W, Hinrichs W, J Mol Biol. 1998 Jun 5;279(2):439-47. PMID:9642048

Page seeded by OCA on Thu Mar 20 09:53:42 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1a6i.gif|left|200px]]<br /><applet load="1a6i" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1a6i.gif|left|200px]]
-caption="1a6i, resolution 2.4&Aring;" />
-'''TET REPRESSOR, CLASS D VARIANT'''<br />
+ {{Structure
+|PDB= 1a6i |SIZE=350|CAPTION= <scene name='initialview01'>1a6i</scene>, resolution 2.4&Aring;
+|SITE= <scene name='pdbsite=TNE:Empty+Tetracycline+Binding+Tunnel'>TNE</scene>
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''TET REPRESSOR, CLASS D VARIANT'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-A6I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Known structural/functional Site: <scene name='pdbsite=TNE:Empty+Tetracycline+Binding+Tunnel'>TNE</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A6I OCA].
+A6I is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A6I OCA].
 ==Reference==
-Conformational changes of the Tet repressor induced by tetracycline trapping., Orth P, Cordes F, Schnappinger D, Hillen W, Saenger W, Hinrichs W, J Mol Biol. 1998 Jun 5;279(2):439-47. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9642048 9642048]
+Conformational changes of the Tet repressor induced by tetracycline trapping., Orth P, Cordes F, Schnappinger D, Hillen W, Saenger W, Hinrichs W, J Mol Biol. 1998 Jun 5;279(2):439-47. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9642048 9642048]
 [[Category: Escherichia coli]]
 [[Category: Single protein]]
@@ Line 23: / Line 32: @@
 [[Category: transcription regulation]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:41:24 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:53:42 2008''