2xhe: Difference between revisions

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-[[Image:2xhe.png|left|200px]]
+==Crystal structure of the Unc18-syntaxin 1 complex from Monosiga brevicollis==
+<StructureSection load='2xhe' size='340' side='right' caption='[[2xhe]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2xhe]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Monosiga_brevicollis Monosiga brevicollis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XHE OCA]. <br>
+</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
+<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xhe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xhe OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xhe RCSB], [http://www.ebi.ac.uk/pdbsum/2xhe PDBsum]</span></td></tr>
+<table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+SNARE protein-driven secretion of neurotransmitters from synaptic vesicles is at the center of neuronal communication. In the absence of the cytosolic protein Munc18-1, synaptic secretion comes to a halt. Although it is believed that Munc18-1 orchestrates SNARE complexes, its mode of action is still a matter of debate. In particular, it has been challenging to clarify the role of a tight Munc18/syntaxin 1 complex, because this interaction interferes strongly with syntaxin's ability to form a SNARE complex. In this complex, two regions of syntaxin, the N-peptide and the remainder in closed conformation, bind to Munc18 simultaneously. Until now, this binary complex has been reported for neuronal tissues only, leading to the hypothesis that it might be a specialization of the neuronal secretion apparatus. Here we aimed, by comparing the core secretion machinery of the unicellular choanoflagellate Monosiga brevicollis with that of animals, to reconstruct the ancestral function of the Munc18/syntaxin1 complex. We found that the Munc18/syntaxin 1 complex from M. brevicollis is structurally and functionally highly similar to the vertebrate complex, suggesting that it constitutes a fundamental step in the reaction pathway toward SNARE assembly. We thus propose that the primordial secretion machinery of the common ancestor of choanoflagellates and animals has been co-opted for synaptic roles during the rise of animals.
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+Primordial neurosecretory apparatus identified in the choanoflagellate Monosiga brevicollis.,Burkhardt P, Stegmann CM, Cooper B, Kloepper TH, Imig C, Varoqueaux F, Wahl MC, Fasshauer D Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):15264-9. Epub 2011 Aug 29. PMID:21876177<ref>PMID:21876177</ref>
-The line below this paragraph, containing "STRUCTURE_2xhe", creates the "Structure Box" on the page.
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-or leave the SCENE parameter empty for the default display.
--->
-{{STRUCTURE_2xhe|  PDB=2xhe  |  SCENE=  }}
-===Crystal structure of the Unc18-syntaxin 1 complex from Monosiga brevicollis===
+From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
+</div>
+== References ==
-<!--
+<references/>
-The line below this paragraph, {{ABSTRACT_PUBMED_21876177}}, adds the Publication Abstract to the page
+__TOC__
-(as it appears on PubMed at http://www.pubmed.gov), where 21876177 is the PubMed ID number.
+</StructureSection>
--->
-{{ABSTRACT_PUBMED_21876177}}
-==About this Structure==
-[[2xhe]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Monosiga_brevicollis Monosiga brevicollis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XHE OCA].
-==Reference==
-<ref group="xtra">PMID:021876177</ref><references group="xtra"/>
 [[Category: Monosiga brevicollis]]
 [[Category: Burkhardt, P.]]