2yjl: Difference between revisions

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-[[Image:2yjl.png|left|200px]]
+==STRUCTURAL CHARACTERIZATION OF A SECRETIN PILOT PROTEIN FROM THE TYPE III SECRETION SYSTEM (T3SS) OF PSEUDOMONAS AERUGINOSA==
+<StructureSection load='2yjl' size='340' side='right' caption='[[2yjl]], [[Resolution|resolution]] 1.81&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2yjl]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YJL OCA]. <br>
+</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
+<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
+<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yjl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yjl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2yjl RCSB], [http://www.ebi.ac.uk/pdbsum/2yjl PDBsum]</span></td></tr>
+<table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Pseudomonas aeruginosa is an opportunistic human pathogen that employs a finely tuned type III secretion system (T3SS) to inject toxins directly into the cytoplasm of target cells. ExsB is a 15.6-kDa protein encoded in a T3SS transcription regulation operon that displays high sequence similarity to YscW, a lipoprotein from Yersinia spp. whose genetic neighborhood also involves a transcriptional regulator, and has been shown to play a role in the stabilization of the outer membrane ring of the T3SS. Here, we show that ExsB is expressed in P. aeruginosa upon induction of the T3SS, and subcellular fractionation studies reveal that it is associated with the outer membrane. The high-resolution crystal structure of ExsB shows that it displays a compact beta-sandwich fold with interdependent beta-sheets. ExsB possesses a large patch of basic residues that could play a role in membrane recognition, and its structure is distinct from that of MxiM, a lipoprotein involved in secretin stabilization in Shigella, as well as from those of Pil lipoproteins involved in pilus biogenesis. These results reveal that small lipoproteins involved in formation of the outer membrane secretin ring display clear structural differences that may be related to the different functions they play in these systems.
-<!--
+Structural Characterization and Membrane Localization of ExsB from the Type III Secretion System (T3SS) of Pseudomonas aeruginosa.,Izore T, Perdu C, Job V, Attree I, Faudry E, Dessen A J Mol Biol. 2011 Oct 14;413(1):236-46. Epub 2011 Aug 4. PMID:21839744<ref>PMID:21839744</ref>
-The line below this paragraph, containing "STRUCTURE_2yjl", creates the "Structure Box" on the page.
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-or leave the SCENE parameter empty for the default display.
--->
-{{STRUCTURE_2yjl|  PDB=2yjl  |  SCENE=  }}
-===STRUCTURAL CHARACTERIZATION OF A SECRETIN PILOT PROTEIN FROM THE TYPE III SECRETION SYSTEM (T3SS) OF PSEUDOMONAS AERUGINOSA===
+From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
+</div>
+== References ==
-<!--
+<references/>
-The line below this paragraph, {{ABSTRACT_PUBMED_21839744}}, adds the Publication Abstract to the page
+__TOC__
-(as it appears on PubMed at http://www.pubmed.gov), where 21839744 is the PubMed ID number.
+</StructureSection>
--->
-{{ABSTRACT_PUBMED_21839744}}
-==About this Structure==
-[[2yjl]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YJL OCA].
-==Reference==
-<ref group="xtra">PMID:021839744</ref><references group="xtra"/>
 [[Category: Pseudomonas aeruginosa]]
 [[Category: Atree, I.]]