2x8u: Difference between revisions

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-[[Image:2x8u.png|left|200px]]
+==SPHINGOMONAS WITTICHII SERINE PALMITOYLTRANSFERASE==
+<StructureSection load='2x8u' size='340' side='right' caption='[[2x8u]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2x8u]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sphingomonas_wittichii Sphingomonas wittichii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X8U OCA]. <br>
+</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=LLP:2-LYSINE(3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHANE)'>LLP</scene></td></tr>
+<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
+<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2x8u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x8u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2x8u RCSB], [http://www.ebi.ac.uk/pdbsum/2x8u PDBsum]</span></td></tr>
+<table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x8/2x8u_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Serine palmitoyltransferase (SPT) catalyses the first step in the de novo biosynthesis of sphingolipids (SLs). It uses a decarboxylative Claisen-like condensation reaction to couple L-serine with palmitoyl-CoA to generate a long-chain base product, 3-ketodihydrosphingosine. SLs are produced by mammals, plants, yeast, and some bacteria, and we have exploited the complete genome sequence of Sphingomonas wittichii to begin a complete analysis of bacterial sphingolipid biosynthesis. Here, we describe the enzymatic characterization of the SPT from this organism and present its high-resolution x-ray structure. Moreover, we identified an open reading frame with high sequence homology to acyl carrier proteins (ACPs) that are common to fatty acid biosynthetic pathways. This small protein was co-expressed with the SPT and we isolated and characterised the apo- and holo-forms of the ACP. Our studies suggest a link between fatty acid and sphingolipid metabolism. (c) 2010 Wiley Periodicals, Inc. Biopolymers 93: 811-822, 2010.
-<!--
+The serine palmitoyltransferase from Sphingomonas wittichii RW1: An interesting link to an unusual acyl carrier protein.,Raman MC, Johnson KA, Clarke DJ, Naismith JH, Campopiano DJ Biopolymers. 2010 Sep;93(9):811-22. PMID:20578000<ref>PMID:20578000</ref>
-The line below this paragraph, containing "STRUCTURE_2x8u", creates the "Structure Box" on the page.
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-or leave the SCENE parameter empty for the default display.
--->
-{{STRUCTURE_2x8u|  PDB=2x8u  |  SCENE=  }}
-===SPHINGOMONAS WITTICHII SERINE PALMITOYLTRANSFERASE===
+From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
+</div>
+== References ==
-<!--
+<references/>
-The line below this paragraph, {{ABSTRACT_PUBMED_20578000}}, adds the Publication Abstract to the page
+__TOC__
-(as it appears on PubMed at http://www.pubmed.gov), where 20578000 is the PubMed ID number.
+</StructureSection>
--->
-{{ABSTRACT_PUBMED_20578000}}
-==About this Structure==
-[[2x8u]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sphingomonas_wittichii Sphingomonas wittichii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X8U OCA].
-==Reference==
-<ref group="xtra">PMID:020578000</ref><references group="xtra"/>
 [[Category: Serine C-palmitoyltransferase]]
 [[Category: Sphingomonas wittichii]]