Molecular Playground/ClyA: Difference between revisions
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==Research on ClyA at UMass Amherst== | ==Research on ClyA at UMass Amherst== | ||
The Chen Lab, in collaboration with the Heuck lab, recently published a paper on [http://www.jbc.org/content/288/43/31042.short, ClyA] assembly. Currently, we are investigating electroosmotic flow and electrophoretic force, the forces that influence polymer translocation through ClyA. We use a technique commonly used for nanopore sensing called electrophysiology which allows us to measure the current passing through the ClyA nanopore. | The Chen Lab, in collaboration with the Heuck lab, recently published a paper on [http://www.jbc.org/content/288/43/31042.short, ClyA] assembly. Currently, we are investigating electroosmotic flow and electrophoretic force, the forces that influence polymer translocation through ClyA. We use a technique commonly used for nanopore sensing called electrophysiology which allows us to measure the current passing through the ClyA nanopore. | ||
==References== | |||
1. Wallace, a J. et al. E. coli hemolysin E (HlyE, ClyA, SheA): X-ray crystal structure of the toxin and observation of membrane pores by electron microscopy. Cell 100, 265–76 (2000). | |||
2. Atkins, a et al. Structure-function relationships of a novel bacterial toxin, hemolysin E. The role of alpha G. J. Biol. Chem. 275, 41150–5 (2000). | |||
3. Mueller, M., Grauschopf, U., Maier, T., Glockshuber, R. & Ban, N. The structure of a cytolytic alpha-helical toxin pore reveals its assembly mechanism. Nature 459, 726–30 (2009). | |||
4. Fahie, M. et al. A non-classical assembly pathway of Escherichia coli pore-forming toxin cytolysin A. J. Biol. Chem. 288, 31042–51 (2013). | |||