Molecular Playground/ClyA: Difference between revisions

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Bib Yang, Monifa Fahie, Michal Harel

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 <scene name='57/571278/Clya_protomer/1'>ClyA protomer</scene>
-The protomer of ClyA reveals slight differences between the monomer and protomer (from the dodecamer). The major conformational changes between the monomer and the protomer are the positions of the <B><font color="blue">N-terminal helix</font></B> and the <B><font color="purple">beta-tongue</font></B>. As ClyA oligomerizes and forms a pore, the N-terminal helix swings to the opposite side of the molecule while the beta-tongue changes its conformation and turns into an alpha-helix that interacts with the lipid bilayer.
+The protomer of ClyA reveals slight differences between the monomer and protomer (from the dodecameric oligomer). The major conformational changes between the monomer and the protomer are the positions of the <B><font color="blue">N-terminal helix</font></B> and the <B><font color="purple">beta-tongue</font></B>. As ClyA oligomerizes and forms a pore, the N-terminal helix swings to the opposite side of the molecule while the beta-tongue changes its conformation and turns into an alpha-helix that interacts with the lipid bilayer.
 <scene name='57/571278/Clya_oligomer/1'>The oligomeric form of ClyA</scene>
 Its crystal structure, [[2WCD]], reveals a dodecamer. Larger [http://pubs.acs.org/doi/abs/10.1021/ja4053398 pores] have been isolated, as well.
 ==Research on ClyA at UMass Amherst==
-The Chen Lab, in collaboration with the Heuck lab, recently published a paper on [http://www.jbc.org/content/288/43/31042.short, ClyA] assembly. Currently, we are investigating electroosmotic flow and electrophoretic force, the forces that influence polymer translocation through ClyA.
+The Chen Lab, in collaboration with the Heuck lab, recently published a paper on [http://www.jbc.org/content/288/43/31042.short, ClyA] assembly. Currently, we are investigating electroosmotic flow and electrophoretic force, the forces that influence polymer translocation through ClyA. We use a technique commonly used for nanopore sensing called electrophysiology which allows us to measure the current passing through the ClyA nanopore.