2kod: Difference between revisions

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-[[Image:2kod.png|left|200px]]
+==A high-resolution NMR structure of the dimeric C-terminal domain of HIV-1 CA==
+<StructureSection load='2kod' size='340' side='right' caption='[[2kod]], [[NMR_Ensembles_of_Models | 30 NMR models]]' scene=''>
+== Structural highlights ==
+[[2kod]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Hiv-1 Hiv-1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOD OCA]. <br>
+<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
+== Publication Abstract from PubMed ==
+Mature HIV-1 particles contain conical-shaped capsids that enclose the viral RNA genome and perform essential functions in the virus life cycle. Previous structural analysis of two- and three-dimensional arrays of the capsid protein (CA) hexamer revealed three interfaces. Here, we present a cryoEM study of a tubular assembly of CA and a high-resolution NMR structure of the CA C-terminal domain (CTD) dimer. In the solution dimer structure, the monomers exhibit different relative orientations compared to previous X-ray structures. The solution structure fits well into the EM density map, suggesting that the dimer interface is retained in the assembled CA. We also identified a CTD-CTD interface at the local three-fold axis in the cryoEM map and confirmed its functional importance by mutagenesis. In the tubular assembly, CA intermolecular interfaces vary slightly, accommodating the asymmetry present in tubes. This provides the necessary plasticity to allow for controlled virus capsid dis/assembly.
-<!--
+Structural convergence between Cryo-EM and NMR reveals intersubunit interactions critical for HIV-1 capsid function.,Byeon IJ, Meng X, Jung J, Zhao G, Yang R, Ahn J, Shi J, Concel J, Aiken C, Zhang P, Gronenborn AM Cell. 2009 Nov 13;139(4):780-90. PMID:19914170<ref>PMID:19914170</ref>
-The line below this paragraph, containing "STRUCTURE_2kod", creates the "Structure Box" on the page.
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-or leave the SCENE parameter empty for the default display.
--->
-{{STRUCTURE_2kod|  PDB=2kod  |  SCENE=  }}
-===A high-resolution NMR structure of the dimeric C-terminal domain of HIV-1 CA===
+From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
+== References ==
+<references/>
-<!--
+__TOC__
-The line below this paragraph, {{ABSTRACT_PUBMED_19914170}}, adds the Publication Abstract to the page
+</StructureSection>
-(as it appears on PubMed at http://www.pubmed.gov), where 19914170 is the PubMed ID number.
+[[Category: Hiv-1]]
--->
-{{ABSTRACT_PUBMED_19914170}}
-==About this Structure==
-[[2kod]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Hiv-1_m:b_hxb2r Hiv-1 m:b_hxb2r]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOD OCA].
-==Reference==
-<ref group="xtra">PMID:019914170</ref><references group="xtra"/>
-[[Category: Hiv-1 m:b_hxb2r]]
 [[Category: Ahn, J.]]
 [[Category: Byeon, I J.L.]]