2ds0: Difference between revisions
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==About this Structure== | ==About this Structure== | ||
2DS0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lumbricus_terrestris Lumbricus terrestris] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DS0 OCA]. | 2DS0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lumbricus_terrestris Lumbricus terrestris] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=AC1:Sia+Binding+Site+For+Residue+B+270'>AC1</scene>, <scene name='pdbsite=AC2:Gal+Binding+Site+For+Residue+B+271'>AC2</scene>, <scene name='pdbsite=AC3:Sia+Binding+Site+For+Residue+A+280'>AC3</scene>, <scene name='pdbsite=AC4:Gal+Binding+Site+For+Residue+A+281'>AC4</scene>, <scene name='pdbsite=AC5:So4+Binding+Site+For+Residue+A+1001'>AC5</scene>, <scene name='pdbsite=AC6:So4+Binding+Site+For+Residue+A+1002'>AC6</scene>, <scene name='pdbsite=AC7:So4+Binding+Site+For+Residue+A+1003'>AC7</scene>, <scene name='pdbsite=AC8:So4+Binding+Site+For+Residue+A+1004'>AC8</scene> and <scene name='pdbsite=AC9:So4+Binding+Site+For+Residue+B+1005'>AC9</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DS0 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: beta-trefoil fold]] | [[Category: beta-trefoil fold]] | ||
[[Category: earthworm lumbricus | [[Category: earthworm lumbricus terrestri]] | ||
[[Category: galactose]] | [[Category: galactose]] | ||
[[Category: in vitro evolution]] | [[Category: in vitro evolution]] | ||
[[Category: sialic acid]] | [[Category: sialic acid]] | ||
[[Category: sugar binding protein]] | |||
[[Category: sugar complex]] | [[Category: sugar complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Mar 14 09:42:06 2008'' | ||
Revision as of 10:42, 14 March 2008
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Crystal structure of the earthworm lectin C-terminal domain mutant in complex with 6'-sialyllactose
OverviewOverview
Sialic acid (Sia) is a typical terminal sugar, which modifies various types of glycoconjugates commonly found in higher animals. Its regulatory roles in diverse biological phenomena are frequently triggered by interaction with Sia-binding lectins. When using natural Sia-binding lectins as probes, however, there have been practical problems concerning their repertoire and availability. Here, we show a rational creation of a Sia-binding lectin based on the strategy 'natural evolution-mimicry', where Sia-binding lectins are engineered by error-prone PCR from a Gal-binding lectin used as a scaffold protein. After selection with fetuin-agarose using a recently reinforced ribosome display system, one of the evolved mutants SRC showed substantial affinity for alpha2-6Sia, which the parental Gal-binding lectin EW29Ch lacked. SRC was found to have additional practical advantages in productivity and in preservation of affinity for Gal. Thus, the developed novel Sia-recognition protein will contribute as useful tools to sialoglycomics.
About this StructureAbout this Structure
2DS0 is a Single protein structure of sequence from Lumbricus terrestris with as ligand. Known structural/functional Sites: , , , , , , , and . Full crystallographic information is available from OCA.
ReferenceReference
Tailoring a novel sialic acid-binding lectin from a ricin-B chain-like galactose-binding protein by natural evolution-mimicry., Yabe R, Suzuki R, Kuno A, Fujimoto Z, Jigami Y, Hirabayashi J, J Biochem. 2007 Mar;141(3):389-99. Epub 2007 Jan 18. PMID:17234683
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