4c4w: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Structure of a rare, non-standard sequence k-turn bound by L7Ae protein== | |||
<StructureSection load='4c4w' size='340' side='right' caption='[[4c4w]], [[Resolution|resolution]] 2.95Å' scene=''> | |||
== Structural highlights == | |||
[[4c4w]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Arcfu Arcfu] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C4W OCA]. <br> | |||
<b>Related:</b> [[4c40|4c40]]<br> | |||
<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br> | |||
== Publication Abstract from PubMed == | |||
Kt-23 from Thelohania solenopsae is a rare RNA kink turn (k-turn) where an adenine replaces the normal guanine at the 2n position. L7Ae is a member of a strongly conserved family of proteins that bind a range of k-turn structures in the ribosome, box C/D and H/ACA small nucleolar RNAs and U4 small nuclear RNA. We have solved the crystal structure of T. solenopsae Kt-23 RNA bound to Archeoglobus fulgidus L7Ae protein at a resolution of 2.95 A. The protein binds in the major groove displayed on the outer face of the k-turn, in a manner similar to complexes with standard k-turn structures. The k-turn adopts a standard N3 class conformation, with a single hydrogen bond from A2b N6 to A2n N3. This contrasts with the structure of the same sequence located in the SAM-I riboswitch, where it adopts an N1 structure, showing the inherent plasticity of k-turn structure. This potentially can affect any tertiary interactions in which the RNA participates. | |||
Structure of a rare non-standard sequence k-turn bound by L7Ae protein.,Huang L, Lilley DM Nucleic Acids Res. 2014 Jan 29. PMID:24482444<ref>PMID:24482444</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
== References == | |||
<references/> | |||
== | __TOC__ | ||
</StructureSection> | |||
[[Category: Arcfu]] | |||
[[Category: Human]] | |||
[[Category: Huang, L.]] | [[Category: Huang, L.]] | ||
[[Category: Lilley, D M.J.]] | [[Category: Lilley, D M.J.]] | ||
Revision as of 08:22, 30 April 2014
Structure of a rare, non-standard sequence k-turn bound by L7Ae proteinStructure of a rare, non-standard sequence k-turn bound by L7Ae protein
Structural highlights4c4w is a 8 chain structure with sequence from Arcfu and Human. Full crystallographic information is available from OCA. Related: 4c40 Publication Abstract from PubMedKt-23 from Thelohania solenopsae is a rare RNA kink turn (k-turn) where an adenine replaces the normal guanine at the 2n position. L7Ae is a member of a strongly conserved family of proteins that bind a range of k-turn structures in the ribosome, box C/D and H/ACA small nucleolar RNAs and U4 small nuclear RNA. We have solved the crystal structure of T. solenopsae Kt-23 RNA bound to Archeoglobus fulgidus L7Ae protein at a resolution of 2.95 A. The protein binds in the major groove displayed on the outer face of the k-turn, in a manner similar to complexes with standard k-turn structures. The k-turn adopts a standard N3 class conformation, with a single hydrogen bond from A2b N6 to A2n N3. This contrasts with the structure of the same sequence located in the SAM-I riboswitch, where it adopts an N1 structure, showing the inherent plasticity of k-turn structure. This potentially can affect any tertiary interactions in which the RNA participates. Structure of a rare non-standard sequence k-turn bound by L7Ae protein.,Huang L, Lilley DM Nucleic Acids Res. 2014 Jan 29. PMID:24482444[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||