2dd8: Difference between revisions
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{{STRUCTURE_2dd8| PDB=2dd8 | SCENE= }} | {{STRUCTURE_2dd8| PDB=2dd8 | SCENE= }} | ||
===Crystal Structure of SARS-CoV Spike Receptor-Binding Domain Complexed with Neutralizing Antibody=== | ===Crystal Structure of SARS-CoV Spike Receptor-Binding Domain Complexed with Neutralizing Antibody=== | ||
{{ABSTRACT_PUBMED_16597622}} | |||
==Function== | |||
[[http://www.uniprot.org/uniprot/SPIKE_CVHSA SPIKE_CVHSA]] S1 attaches the virion to the cell membrane by interacting with human ACE2 and CLEC4M/DC-SIGNR, initiating the infection. Binding to the receptor and internalization of the virus into the endosomes of the host cell probably induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. S2 is a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. | |||
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==About this Structure== | ==About this Structure== | ||
[[2dd8]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DD8 OCA]. | |||
==Reference== | ==Reference== | ||
<ref group="xtra">PMID: | <ref group="xtra">PMID:016597622</ref><references group="xtra"/><references/> | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: | [[Category: Sars coronavirus]] | ||
[[Category: Dimitrov, D S.]] | [[Category: Dimitrov, D S.]] | ||
[[Category: Feng, Y.]] | [[Category: Feng, Y.]] | ||
| Line 34: | Line 21: | ||
[[Category: Zhu, Z Y.]] | [[Category: Zhu, Z Y.]] | ||
[[Category: Antibody]] | [[Category: Antibody]] | ||
[[Category: Epitope]] | [[Category: Epitope]] | ||
[[Category: Immune system-viral protein complex]] | |||
[[Category: Inhibitor]] | [[Category: Inhibitor]] | ||
[[Category: S protein]] | [[Category: S protein]] | ||
[[Category: Sar]] | [[Category: Sar]] | ||
[[Category: Vaccine]] | [[Category: Vaccine]] | ||
Revision as of 11:46, 23 April 2014
Crystal Structure of SARS-CoV Spike Receptor-Binding Domain Complexed with Neutralizing AntibodyCrystal Structure of SARS-CoV Spike Receptor-Binding Domain Complexed with Neutralizing Antibody
Template:ABSTRACT PUBMED 16597622
FunctionFunction
[SPIKE_CVHSA] S1 attaches the virion to the cell membrane by interacting with human ACE2 and CLEC4M/DC-SIGNR, initiating the infection. Binding to the receptor and internalization of the virus into the endosomes of the host cell probably induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. S2 is a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
About this StructureAbout this Structure
2dd8 is a 3 chain structure with sequence from Homo sapiens and Sars coronavirus. Full crystallographic information is available from OCA.
ReferenceReference
- ↑ Prabakaran P, Gan J, Feng Y, Zhu Z, Choudhry V, Xiao X, Ji X, Dimitrov DS. Structure of severe acute respiratory syndrome coronavirus receptor-binding domain complexed with neutralizing antibody. J Biol Chem. 2006 Jun 9;281(23):15829-36. Epub 2006 Apr 5. PMID:16597622 doi:10.1074/jbc.M600697200