Proteinase: Difference between revisions
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<StructureSection load='2duj' size='340' side='right' caption='E. coli proteinase K complex with lactoferrin peptide and Ca+2 (PDB code [[2duj]])' scene=''> | <StructureSection load='2duj' size='340' side='right' caption='E. coli proteinase K complex with lactoferrin peptide and Ca+2 (PDB code [[2duj]])' scene=''> | ||
'''Proteinase''' (PRO) are enzymes which hydrolyze peptide bonds. They are classified by the amino acid site of their cleavage or by the pH at which they are active. PRO B is a serine protease. For more details on PRO B see [[Streptomyces griseus proteinase B]]. PRO A is a carboxylproteinase. PRO K is a serine protease which cleaves proteins preferentially after hydrophobic residues. Calcium ions contribute to the stability of the enzyme. PRO is active over a wide pH range and is used in molecular biology to inactivate nucleases from preparations of DNA or RNA. PRO is used in the partial proteolysis of lactoferrin into its N- and C-lobe. The two lobes of lactoferrin have different antimicrobial and antifungal properties. PRO K can digest hair (keratin). | '''Proteinase''' (PRO) are enzymes which hydrolyze peptide bonds. They are classified by the amino acid site of their cleavage or by the pH at which they are active. PRO B is a serine protease. For more details on PRO B see [[Streptomyces griseus proteinase B]]. PRO A is a carboxylproteinase. PRO K is a serine protease which cleaves proteins preferentially after hydrophobic residues. Calcium ions contribute to the stability of the enzyme. PRO K is active over a wide pH range and is used in molecular biology to inactivate nucleases from preparations of DNA or RNA. PRO K is used in the partial proteolysis of lactoferrin into its N- and C-lobe. The two lobes of lactoferrin have different antimicrobial and antifungal properties. PRO K can digest hair (keratin). | ||
==3D structures of proteinase== | ==3D structures of proteinase== | ||
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'''PRO B''' | '''PRO B''' | ||
[[ | [[3sgb]] – SgPRO + turkey ovomucoid inhibitor<br /> | ||
[[1sgp]], [[1sgq]], [[1sgr]], [[1cso]], [[1ct0]], [[1ct2]], [[1ct4]], [[1ds2]], [[2sgp]], [[2nu3]], [[2nu4]] – SgPRO + turkey ovomucoid inhibitor (mutant)<br /> | [[1sgp]], [[1sgq]], [[1sgr]], [[1cso]], [[1ct0]], [[1ct2]], [[1ct4]], [[1ds2]], [[2sgp]], [[2nu3]], [[2nu4]] – SgPRO + turkey ovomucoid inhibitor (mutant)<br /> | ||
[[4sgb]] - SgPRO + potato inhibitor | [[4sgb]] - SgPRO + potato inhibitor | ||