9gaf: Difference between revisions
New page: left|200px<br /><applet load="9gaf" size="450" color="white" frame="true" align="right" spinBox="true" caption="9gaf, resolution 1.9Å" /> '''PRECURSOR OF THE W11F... |
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[[Image:9gaf.gif|left|200px]]<br /><applet load="9gaf" size=" | [[Image:9gaf.gif|left|200px]]<br /><applet load="9gaf" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="9gaf, resolution 1.9Å" /> | caption="9gaf, resolution 1.9Å" /> | ||
'''PRECURSOR OF THE W11F MUTANT GLYCOSYLASPARAGINASE FROM FLAVOBACTERIUM MENINGOSEPTICUM'''<br /> | '''PRECURSOR OF THE W11F MUTANT GLYCOSYLASPARAGINASE FROM FLAVOBACTERIUM MENINGOSEPTICUM'''<br /> | ||
==Overview== | ==Overview== | ||
A variety of proteins, including glycosylasparaginase, have recently been | A variety of proteins, including glycosylasparaginase, have recently been found to activate functions by self-catalyzed peptide bond rearrangements from single-chain precursors. Here we present the 1.9 A crystal structures of glycosylasparaginase precursors that are able to autoproteolyze via an N --> O acyl shift. Several conserved residues are aligned around the scissile peptide bond that is in a highly strained trans peptide bond configuration. The structure illustrates how a nucleophilic side chain may attack the scissile peptide bond at the immediate upstream backbone carbonyl and provides an understanding of the structural basis for peptide bond cleavage via an N --> O or N --> S acyl shift that is used by various groups of intramolecular autoprocessing proteins. | ||
==About this Structure== | ==About this Structure== | ||
9GAF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica] with GLY as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.26 3.5.1.26] Full crystallographic information is available from [http:// | 9GAF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica] with <scene name='pdbligand=GLY:'>GLY</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.26 3.5.1.26] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9GAF OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase]] | [[Category: N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Guo, H | [[Category: Guo, H C.]] | ||
[[Category: Xu, Q.]] | [[Category: Xu, Q.]] | ||
[[Category: GLY]] | [[Category: GLY]] | ||
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[[Category: precursor]] | [[Category: precursor]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:18:30 2008'' | ||
