2bsx: Difference between revisions
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Revision as of 17:42, 30 October 2007
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CRYSTAL STRUCTURE OF THE PLASMODIUM FALCIPARUM PURINE NUCLEOSIDE PHOSPHORYLASE COMPLEXED WITH INOSINE
OverviewOverview
Purine metabolism in the parasite Plasmodium has been identified as a, promising target for antimalarial therapies. Purine nucleoside, phosphorylase (PNP) is part of a salvage pathway for the biosynthesis of, purines, which are essential for parasite survival. Two crystal structures, of PNP from Plasmodium falciparum (PfPNP) in two space groups, each with a, single subunit in the asymmetric unit, are described here. One structure, refined to 2.4 A, has an empty nucleoside-binding site and a sulfate ion, bound in the phosphate-binding pocket. The second structure, refined to, 2.0 A, has the substrate inosine bound to the active centre. Structure, comparison reveals alterations in the active site upon ligand binding. The, new structures presented here specifically highlight the likely roles ... [(full description)]
About this StructureAbout this Structure
2BSX is a [Single protein] structure of sequence from [Plasmodium falciparum] with NOS as [ligand]. Active as [Purine-nucleoside phosphorylase], with EC number [2.4.2.1]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
Structures of Plasmodium falciparum purine nucleoside phosphorylase complexed with sulfate and its natural substrate inosine., Schnick C, Robien MA, Brzozowski AM, Dodson EJ, Murshudov GN, Anderson L, Luft JR, Mehlin C, Hol WG, Brannigan JA, Wilkinson AJ, Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1245-54. Epub 2005, Aug 16. PMID:16131758
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