5cgt: Difference between revisions

Revision as of 20:15, 21 February 2008

MALTOTRIOSE COMPLEX OF PRECONDITIONED CYCLODEXTRIN GLYCOSYLTRANSFERASE MUTANT

OverviewOverview

Bacterial cyclodextrin glycosyltransferases use starch to produce cyclic maltooligosaccharides (cyclodextrins) which are of interest in various applications. The cyclization reaction gives rise to a spectrum of ring sizes consisting of predominantly six to eight glucosyl units. Using the enzyme from Bacillus circulans strain no. 8, binding studies have been performed with several substrates and analogues. The observed binding modes differ in detail, but agree in general with data on homologous enzymes. Based on these binding studies, two mutations were designed that changed the production spectrum from the predominant product beta-cyclodextrin of the wild-type enzyme towards gamma-cyclodextrin, which is of practical interest because it is rare and can encapsulate larger nonpolar compounds.

About this StructureAbout this Structure

5CGT is a Single protein structure of sequence from Bacillus circulans with and as ligands. Active as Cyclomaltodextrin glucanotransferase, with EC number 2.4.1.19 Full crystallographic information is available from OCA.

ReferenceReference

Substrate binding to a cyclodextrin glycosyltransferase and mutations increasing the gamma-cyclodextrin production., Parsiegla G, Schmidt AK, Schulz GE, Eur J Biochem. 1998 Aug 1;255(3):710-7. PMID:9738912

Page seeded by OCA on Thu Feb 21 19:14:56 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:5cgt.gif|left|200px]]<br /><applet load="5cgt" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:5cgt.gif|left|200px]]<br /><applet load="5cgt" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="5cgt, resolution 2.5&Aring;" />
 '''MALTOTRIOSE COMPLEX OF PRECONDITIONED CYCLODEXTRIN GLYCOSYLTRANSFERASE MUTANT'''<br />
 ==Overview==
-Bacterial cyclodextrin glycosyltransferases use starch to produce cyclic, maltooligosaccharides (cyclodextrins) which are of interest in various, applications. The cyclization reaction gives rise to a spectrum of ring, sizes consisting of predominantly six to eight glucosyl units. Using the, enzyme from Bacillus circulans strain no. 8, binding studies have been, performed with several substrates and analogues. The observed binding, modes differ in detail, but agree in general with data on homologous, enzymes. Based on these binding studies, two mutations were designed that, changed the production spectrum from the predominant product, beta-cyclodextrin of the wild-type enzyme towards gamma-cyclodextrin, which is of practical interest because it is rare and can encapsulate, larger nonpolar compounds.
+Bacterial cyclodextrin glycosyltransferases use starch to produce cyclic maltooligosaccharides (cyclodextrins) which are of interest in various applications. The cyclization reaction gives rise to a spectrum of ring sizes consisting of predominantly six to eight glucosyl units. Using the enzyme from Bacillus circulans strain no. 8, binding studies have been performed with several substrates and analogues. The observed binding modes differ in detail, but agree in general with data on homologous enzymes. Based on these binding studies, two mutations were designed that changed the production spectrum from the predominant product beta-cyclodextrin of the wild-type enzyme towards gamma-cyclodextrin, which is of practical interest because it is rare and can encapsulate larger nonpolar compounds.
 ==About this Structure==
-CGT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans] with GLC and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=5CGT OCA].
+CGT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans] with <scene name='pdbligand=GLC:'>GLC</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CGT OCA].
 ==Reference==
@@ Line 15: / Line 15: @@
 [[Category: Single protein]]
 [[Category: Parsiegla, G.]]
-[[Category: Schulz, G.E.]]
+[[Category: Schulz, G E.]]
 [[Category: CA]]
 [[Category: GLC]]
@@ Line 22: / Line 22: @@
 [[Category: starch degradation]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:25:47 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:14:56 2008''