4tss: Difference between revisions
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==Overview== | ==Overview== | ||
The structure of toxic shock syndrome toxin-1 (TSST-1), the causative | The structure of toxic shock syndrome toxin-1 (TSST-1), the causative agent in toxic shock syndrome, has been determined in three crystal forms. The three structural models have been refined to R-factors of 0.154, 0.150, and 0.198 at resolutions of 2.05 A, 2.90 A, and 2.75 A, respectively. One crystal form of TSST-1 contains a zinc ion bound between two symmetry-related molecules. Although not required for biological activity, zinc dramatically potentiates the mitogenicity of TSST-1 at very low concentrations. In addition, the structure of the tetramutant TSST-1H [T69I, Y80W, E132K, I140T], which is nonmitogenic and does not amplify endotoxin shock, has been determined and refined in a fourth crystal form (R-factor = 0.173 to 1.9 A resolution). | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Staphylococcus aureus]] | [[Category: Staphylococcus aureus]] | ||
[[Category: Cook, W | [[Category: Cook, W J.]] | ||
[[Category: Dinges, M | [[Category: Dinges, M M.]] | ||
[[Category: Earhart, C | [[Category: Earhart, C A.]] | ||
[[Category: Mitchell, D | [[Category: Mitchell, D T.]] | ||
[[Category: Ohlendorf, D | [[Category: Ohlendorf, D H.]] | ||
[[Category: Prasad, G | [[Category: Prasad, G S.]] | ||
[[Category: Radhakrishnan, R.]] | [[Category: Radhakrishnan, R.]] | ||
[[Category: Schlivert, P | [[Category: Schlivert, P M.]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: signal]] | [[Category: signal]] | ||
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[[Category: toxin]] | [[Category: toxin]] | ||
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Revision as of 20:14, 21 February 2008
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TOXIC SHOCK SYNDROME TOXIN-1: TETRAGONAL P4(1)2(1)2 CRYSTAL FORM
OverviewOverview
The structure of toxic shock syndrome toxin-1 (TSST-1), the causative agent in toxic shock syndrome, has been determined in three crystal forms. The three structural models have been refined to R-factors of 0.154, 0.150, and 0.198 at resolutions of 2.05 A, 2.90 A, and 2.75 A, respectively. One crystal form of TSST-1 contains a zinc ion bound between two symmetry-related molecules. Although not required for biological activity, zinc dramatically potentiates the mitogenicity of TSST-1 at very low concentrations. In addition, the structure of the tetramutant TSST-1H [T69I, Y80W, E132K, I140T], which is nonmitogenic and does not amplify endotoxin shock, has been determined and refined in a fourth crystal form (R-factor = 0.173 to 1.9 A resolution).
About this StructureAbout this Structure
4TSS is a Single protein structure of sequence from Staphylococcus aureus with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Refined structures of three crystal forms of toxic shock syndrome toxin-1 and of a tetramutant with reduced activity., Prasad GS, Radhakrishnan R, Mitchell DT, Earhart CA, Dinges MM, Cook WJ, Schlievert PM, Ohlendorf DH, Protein Sci. 1997 Jun;6(6):1220-7. PMID:9194182
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