EPSP synthase: Difference between revisions
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<StructureSection load='1eps' size='350' side='right' caption='Structure of EPSP synthase (PDB entry [[1eps]])' scene=''> | <StructureSection load='1eps' size='350' side='right' caption='Structure of EPSP synthase (PDB entry [[1eps]])' scene=''> | ||
5-enolpyruvylshikimate 3-phosphate (EPSP) synthase is a key enzyme for the biosynthesis of aromatic amino acids in plants and many microbes. Consequently, it is a target for drugs and herbicides. EPSP synthase catalyzes the addition of phosphoenol pyruvate (PEP) to shikimate-3-phosphate, generating 5-enolpyruvylshikimate-3-phosphate, which is a precursor for phenylalanine and tyrosine. | '''5-enolpyruvylshikimate 3-phosphate (EPSP) synthase''' is a key enzyme for the biosynthesis of aromatic amino acids in plants and many microbes. Consequently, it is a target for drugs and herbicides. EPSP synthase catalyzes the addition of phosphoenol pyruvate (PEP) to shikimate-3-phosphate, generating 5-enolpyruvylshikimate-3-phosphate, which is a precursor for phenylalanine and tyrosine. | ||
The enzyme has <scene name='57/570585/Two_domains/1'>two domains</scene>, with the active site found in the interdomain cleft. There is a substantial structural change upon substrate binding, resulting in a <scene name='57/570585/Closed_formation/1'>closed formation</scene>. Glyphosate (also known as Roundup) occupies the binding site of the second substrate, phosphoenol pyruvate. | The enzyme has <scene name='57/570585/Two_domains/1'>two domains</scene>, with the active site found in the interdomain cleft. There is a substantial structural change upon substrate binding, resulting in a <scene name='57/570585/Closed_formation/1'>closed formation</scene>. Glyphosate (also known as Roundup) occupies the binding site of the second substrate, phosphoenol pyruvate. | ||
===3D structures of EPSP synthase === | |||
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | |||
[[1eps]] – EcEPSP – ''Escherichia coli''<br /> | |||
[[1p88]], [[1p89]] - EcEPSP N terminal - NMR<br /> | |||
[[1rf5]] - SpEPSP – ''Streptococcus pneumoniae''<br /> | |||
[[2bjb]], [[2o15]] – MtEPSP – ''Mycobacterium tuberculosis''<br /> | |||
[[2gg4]] – AgEPSP – ''Agrobacterium''<br /> | |||
[[3roi]], [[3tr1]], [[4gfp]] – CbEPSP – ''Coxiella burnetii''<br /> | |||
[[3ti2]] - VcEPSP N terminal – ''Vibrio cholerae''<br /> | |||
===EPSP synthase binary complex=== | |||
[[1g6t]] - EcEPSP + S3P <br /> | |||
[[1mi4]], [[2qfq]], [[2qfs]], [[3fjx]], [[3fk0]] - EcEPSP (mutant) + S3P <br /> | |||
[[1q36]] - EcEPSP (mutant) + S3P derivative<br /> | |||
[[1x8r]], [[1x8t]] - EcEPSP + intermediate <br /> | |||
[[2aa9]] – EcEPSP + shikimate<br /> | |||
[[2pq9]] - EcEPSP + intermediate <br /> | |||
[[1rf4]] - SpEPSP + intermediate <br /> | |||
[[2o0b]], [[2o0d]] - MtEPSP + S3P <br /> | |||
[[2gg6]] - AgEPSP + S3P <br /> | |||
[[2pqb]], [[2pqc]], [[2pqd]] - AgEPSP + intermediate <br /> | |||
[[2o0x]] - MtEPSP + intermediate <br /> | |||
[[2o0z]] - MtEPSP + EPS <br /> | |||
[[4egr]] - CbEPSP + phosphoenolpyruvate<br /> | |||
===EPSP synthase ternary complex=== | |||
[[1g6p]] - EcEPSP + S3P + glyphosate<br /> | |||
[[2qft]], [[2qfu]], [[3fjz]], [[3fk1]] - EcEPSP (mutant) + S3P + glyphosate<br /> | |||
[[2aay]] - EcEPSP + shikimate + glyphosate<br /> | |||
[[1rf6]] - SpEPSP + S3P + glyphosate<br /> | |||
[[2gga]] - AgEPSP + S3P + glyphosate<br /> | |||
[[4ggd]] - AgEPSP (mutant) + S3P + glyphosate<br /> | |||
[[2o0e]] - MtEPSP + S3P + phosphoenolpyruvate<br /> | |||
[[3nvs]] - VcEPSP + S3P + glyphosate <br /> | |||
[[3slh]] - CbEPSP + S3P + glyphosate<br /> | |||
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</StructureSection> | </StructureSection> | ||
[[Category:Topic Page]] | |||
Revision as of 12:55, 12 March 2014
Structure of EPSP SynthaseStructure of EPSP Synthase
5-enolpyruvylshikimate 3-phosphate (EPSP) synthase is a key enzyme for the biosynthesis of aromatic amino acids in plants and many microbes. Consequently, it is a target for drugs and herbicides. EPSP synthase catalyzes the addition of phosphoenol pyruvate (PEP) to shikimate-3-phosphate, generating 5-enolpyruvylshikimate-3-phosphate, which is a precursor for phenylalanine and tyrosine. The enzyme has , with the active site found in the interdomain cleft. There is a substantial structural change upon substrate binding, resulting in a . Glyphosate (also known as Roundup) occupies the binding site of the second substrate, phosphoenol pyruvate. 3D structures of EPSP synthaseUpdated on 05-May-2025 1eps – EcEPSP – Escherichia coli 1p88, 1p89 - EcEPSP N terminal - NMR EPSP synthase binary complex1g6t - EcEPSP + S3P 1mi4, 2qfq, 2qfs, 3fjx, 3fk0 - EcEPSP (mutant) + S3P EPSP synthase ternary complex1g6p - EcEPSP + S3P + glyphosate 2qft, 2qfu, 3fjz, 3fk1 - EcEPSP (mutant) + S3P + glyphosate
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