408d: Difference between revisions
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==Overview== | ==Overview== | ||
Polyamide dimers containing three types of aromatic rings-pyrrole, imidazole, and hydroxypyrrole-afford a small-molecule recognition code | Polyamide dimers containing three types of aromatic rings-pyrrole, imidazole, and hydroxypyrrole-afford a small-molecule recognition code that discriminates among all four Watson-Crick base pairs in the minor groove. The crystal structure of a specific polyamide dimer-DNA complex establishes the structural basis for distinguishing T.A from A.T base pairs. Specificity for the T.A base pair is achieved by means of distinct hydrogen bonds between pairs of substituted pyrroles on the ligand and the O2 of thymine and N3 of adenine. In addition, shape-selective recognition of an asymmetric cleft between the thymine-O2 and the adenine-C2 was observed. Although hitherto similarities among the base pairs in the minor groove have been emphasized, the structure illustrates differences that allow specific minor groove recognition. | ||
==About this Structure== | ==About this Structure== | ||
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A structural basis for recognition of A.T and T.A base pairs in the minor groove of B-DNA., Kielkopf CL, White S, Szewczyk JW, Turner JM, Baird EE, Dervan PB, Rees DC, Science. 1998 Oct 2;282(5386):111-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9756473 9756473] | A structural basis for recognition of A.T and T.A base pairs in the minor groove of B-DNA., Kielkopf CL, White S, Szewczyk JW, Turner JM, Baird EE, Dervan PB, Rees DC, Science. 1998 Oct 2;282(5386):111-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9756473 9756473] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Baird, E | [[Category: Baird, E E.]] | ||
[[Category: Dervan, P | [[Category: Dervan, P B.]] | ||
[[Category: Kielkopf, C | [[Category: Kielkopf, C L.]] | ||
[[Category: Rees, D | [[Category: Rees, D C.]] | ||
[[Category: Szewczyk, J | [[Category: Szewczyk, J W.]] | ||
[[Category: Turner, J | [[Category: Turner, J M.]] | ||
[[Category: White, S.]] | [[Category: White, S.]] | ||
[[Category: IPY]] | [[Category: IPY]] | ||
[[Category: drug in the dna minor groove]] | [[Category: drug in the dna minor groove]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:11:56 2008'' | ||
Revision as of 20:11, 21 February 2008
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STRUCTURAL BASIS FOR RECOGNITION OF A-T AND T-A BASE PAIRS IN THE MINOR GROOVE OF B-DNA
OverviewOverview
Polyamide dimers containing three types of aromatic rings-pyrrole, imidazole, and hydroxypyrrole-afford a small-molecule recognition code that discriminates among all four Watson-Crick base pairs in the minor groove. The crystal structure of a specific polyamide dimer-DNA complex establishes the structural basis for distinguishing T.A from A.T base pairs. Specificity for the T.A base pair is achieved by means of distinct hydrogen bonds between pairs of substituted pyrroles on the ligand and the O2 of thymine and N3 of adenine. In addition, shape-selective recognition of an asymmetric cleft between the thymine-O2 and the adenine-C2 was observed. Although hitherto similarities among the base pairs in the minor groove have been emphasized, the structure illustrates differences that allow specific minor groove recognition.
About this StructureAbout this Structure
408D is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
A structural basis for recognition of A.T and T.A base pairs in the minor groove of B-DNA., Kielkopf CL, White S, Szewczyk JW, Turner JM, Baird EE, Dervan PB, Rees DC, Science. 1998 Oct 2;282(5386):111-5. PMID:9756473
Page seeded by OCA on Thu Feb 21 19:11:56 2008