3vub: Difference between revisions

Revision as of 20:11, 21 February 2008

CCDB, A TOPOISOMERASE POISON FROM E. COLI

OverviewOverview

The crystal structure of CcdB, a protein that poisons Escherichia coli gyrase, was determined in three crystal forms. The protein consists of a five-stranded antiparallel beta-pleated sheet followed by a C-terminal alpha-helix. In one of the loops of the sheet, a second small three-stranded antiparallel beta-sheet is inserted that sticks out of the molecule as a wing. This wing contains the LysC proteolytic cleavage site that is protected by CcdA and, therefore, forms a likely CcdA recognition site. A dimer is formed by sheet extension and by extensive hydrophobic contacts involving three of the five methionine residues and the C terminus of the alpha-helix. The surface of the dimer on the side of the alpha-helix is overall negatively charged, while the opposite side as well as the wing sheet is dominated by positive charges. We propose that the CcdB dimer binds into the central hole of the 59 kDa N-terminal fragment of GyrA, after disruption of the head dimer interface of GyrA.

About this StructureAbout this Structure

3VUB is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of CcdB, a topoisomerase poison from E. coli., Loris R, Dao-Thi MH, Bahassi EM, Van Melderen L, Poortmans F, Liddington R, Couturier M, Wyns L, J Mol Biol. 1999 Jan 29;285(4):1667-77. PMID:9917404

Page seeded by OCA on Thu Feb 21 19:11:49 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:3vub.gif|left|200px]]<br /><applet load="3vub" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:3vub.gif|left|200px]]<br /><applet load="3vub" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="3vub, resolution 1.4&Aring;" />
 '''CCDB, A TOPOISOMERASE POISON FROM E. COLI'''<br />
 ==Overview==
-The crystal structure of CcdB, a protein that poisons Escherichia coli, gyrase, was determined in three crystal forms. The protein consists of a, five-stranded antiparallel beta-pleated sheet followed by a C-terminal, alpha-helix. In one of the loops of the sheet, a second small, three-stranded antiparallel beta-sheet is inserted that sticks out of the, molecule as a wing. This wing contains the LysC proteolytic cleavage site, that is protected by CcdA and, therefore, forms a likely CcdA recognition, site. A dimer is formed by sheet extension and by extensive hydrophobic, contacts involving three of the five methionine residues and the C, terminus of the alpha-helix. The surface of the dimer on the side of the, alpha-helix is overall negatively charged, while the opposite side as well, as the wing sheet is dominated by positive charges. We propose that the, CcdB dimer binds into the central hole of the 59 kDa N-terminal fragment, of GyrA, after disruption of the head dimer interface of GyrA.
+The crystal structure of CcdB, a protein that poisons Escherichia coli gyrase, was determined in three crystal forms. The protein consists of a five-stranded antiparallel beta-pleated sheet followed by a C-terminal alpha-helix. In one of the loops of the sheet, a second small three-stranded antiparallel beta-sheet is inserted that sticks out of the molecule as a wing. This wing contains the LysC proteolytic cleavage site that is protected by CcdA and, therefore, forms a likely CcdA recognition site. A dimer is formed by sheet extension and by extensive hydrophobic contacts involving three of the five methionine residues and the C terminus of the alpha-helix. The surface of the dimer on the side of the alpha-helix is overall negatively charged, while the opposite side as well as the wing sheet is dominated by positive charges. We propose that the CcdB dimer binds into the central hole of the 59 kDa N-terminal fragment of GyrA, after disruption of the head dimer interface of GyrA.
 ==About this Structure==
-VUB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with CL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=3VUB OCA].
+VUB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VUB OCA].
 ==Reference==
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 [[Category: Escherichia coli]]
 [[Category: Single protein]]
-[[Category: Bahasi, E.M.]]
+[[Category: Bahasi, E M.]]
 [[Category: Couturier, M.]]
-[[Category: Dao-Thi, M.H.]]
+[[Category: Dao-Thi, M H.]]
 [[Category: Liddington, R.]]
 [[Category: Loris, R.]]
-[[Category: Melderen, L.Van.]]
+[[Category: Melderen, L Van.]]
 [[Category: Poortmans, F.]]
 [[Category: Wyns, L.]]
@@ Line 26: / Line 26: @@
 [[Category: topoisomerase poison]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:00:45 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:11:49 2008''