3kar: Difference between revisions

Revision as of 20:09, 21 February 2008

THE MOTOR DOMAIN OF KINESIN-LIKE PROTEIN KAR3, A SACCHAROMYCES CEREVISIAE KINESIN-RELATED PROTEIN

OverviewOverview

The kinesin family of motor proteins, which contain a conserved motor domain of approximately 350 amino acids, generate movement against microtubules. Over 90 members of this family have been identified, including motors that move toward the minus or plus end of microtubules. The Kar3 protein from Saccharomyces cerevisiae is a minus end-directed kinesin family member that is involved in both nuclear fusion, or karyogamy, and mitosis. The Kar3 protein is 729 residues in length with the motor domain located in the C-terminal 347 residues. Recently, the three-dimensional structures of two kinesin family members have been reported. These structures include the motor domains of the plus end-directed kinesin heavy chain [Kull, F. J., et al. (1996) Nature 380, 550-555] and the minus end-directed Ncd [Sablin, E. P., et al. (1996) Nature 380, 555-559]. We now report the structure of the Kar3 protein complexed with Mg.ADP obtained from crystallographic data to 2.3 A. The structure is similar to those of the earlier kinesin family members, but shows differences as well, most notably in the length of helix alpha 4, a helix which is believed to be involved in conformational changes during the hydrolysis cycle.

About this StructureAbout this Structure

3KAR is a Single protein structure of sequence from Saccharomyces cerevisiae with and as ligands. Full crystallographic information is available from OCA.

ReferenceReference

X-ray crystal structure of the yeast Kar3 motor domain complexed with Mg.ADP to 2.3 A resolution., Gulick AM, Song H, Endow SA, Rayment I, Biochemistry. 1998 Feb 17;37(7):1769-76. PMID:9485302

Page seeded by OCA on Thu Feb 21 19:09:55 2008

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OCA

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-[[Image:3kar.jpg|left|200px]]<br /><applet load="3kar" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:3kar.jpg|left|200px]]<br /><applet load="3kar" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="3kar, resolution 2.3&Aring;" />
 '''THE MOTOR DOMAIN OF KINESIN-LIKE PROTEIN KAR3, A SACCHAROMYCES CEREVISIAE KINESIN-RELATED PROTEIN'''<br />
 ==Overview==
-The kinesin family of motor proteins, which contain a conserved motor, domain of approximately 350 amino acids, generate movement against, microtubules. Over 90 members of this family have been identified, including motors that move toward the minus or plus end of microtubules., The Kar3 protein from Saccharomyces cerevisiae is a minus end-directed, kinesin family member that is involved in both nuclear fusion, or, karyogamy, and mitosis. The Kar3 protein is 729 residues in length with, the motor domain located in the C-terminal 347 residues. Recently, the, three-dimensional structures of two kinesin family members have been, reported. These structures include the motor domains of the plus, end-directed kinesin heavy chain [Kull, F. J., et al. (1996) Nature 380, 550-555] and the minus end-directed Ncd [Sablin, E. P., et al. (1996), Nature 380, 555-559]. We now report the structure of the Kar3 protein, complexed with Mg.ADP obtained from crystallographic data to 2.3 A. The, structure is similar to those of the earlier kinesin family members, but, shows differences as well, most notably in the length of helix alpha 4, a, helix which is believed to be involved in conformational changes during, the hydrolysis cycle.
+The kinesin family of motor proteins, which contain a conserved motor domain of approximately 350 amino acids, generate movement against microtubules. Over 90 members of this family have been identified, including motors that move toward the minus or plus end of microtubules. The Kar3 protein from Saccharomyces cerevisiae is a minus end-directed kinesin family member that is involved in both nuclear fusion, or karyogamy, and mitosis. The Kar3 protein is 729 residues in length with the motor domain located in the C-terminal 347 residues. Recently, the three-dimensional structures of two kinesin family members have been reported. These structures include the motor domains of the plus end-directed kinesin heavy chain [Kull, F. J., et al. (1996) Nature 380, 550-555] and the minus end-directed Ncd [Sablin, E. P., et al. (1996) Nature 380, 555-559]. We now report the structure of the Kar3 protein complexed with Mg.ADP obtained from crystallographic data to 2.3 A. The structure is similar to those of the earlier kinesin family members, but shows differences as well, most notably in the length of helix alpha 4, a helix which is believed to be involved in conformational changes during the hydrolysis cycle.
 ==About this Structure==
-KAR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with MG and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=3KAR OCA].
+KAR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KAR OCA].
 ==Reference==
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 [[Category: Single protein]]
 [[Category: Endow, S.]]
-[[Category: Gulick, A.M.]]
+[[Category: Gulick, A M.]]
 [[Category: Rayment, I.]]
 [[Category: Song, H.]]
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 [[Category: p-loop]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:48:24 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:09:55 2008''