3u1m: Difference between revisions
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{{STRUCTURE_3u1m| PDB=3u1m | SCENE= }} | {{STRUCTURE_3u1m| PDB=3u1m | SCENE= }} | ||
===Structure of the mRNA splicing complex component Cwc2=== | ===Structure of the mRNA splicing complex component Cwc2=== | ||
{{ABSTRACT_PUBMED_21957909}} | |||
==Function== | |||
[[http://www.uniprot.org/uniprot/CWC2_YEAST CWC2_YEAST]] Involved in the first step of pre-mRNA splicing. Required for cell growth and cell cycle control. Plays a role in the levels of the U1, U4, U5 and U6 snRNAs and the maintenance of the U4/U6 snRNA complex. May provide the link between the "nineteen complex" NTC spliceosome protein complex and the spliceosome through the U6 snRNA. Associates predominantly with U6 snRNAs in assembled active spliceosomes. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Binds also to U1, U4, U5 and U6 snRNAs and to pre-mRNAs, in vitro. Is not required for the Prp2-mediated remodeling of the activated spliceosome.<ref>PMID:19435883</ref> <ref>PMID:22246180</ref> | |||
==About this Structure== | ==About this Structure== | ||
[[3u1m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/ | [[3u1m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U1M OCA]. | ||
[[Category: | |||
==Reference== | |||
<ref group="xtra">PMID:021957909</ref><references group="xtra"/><references/> | |||
[[Category: Baker's yeast]] | |||
[[Category: Li, W.]] | [[Category: Li, W.]] | ||
[[Category: Lu, G.]] | [[Category: Lu, G.]] | ||
Revision as of 12:17, 5 February 2014
Structure of the mRNA splicing complex component Cwc2Structure of the mRNA splicing complex component Cwc2
Template:ABSTRACT PUBMED 21957909
FunctionFunction
[CWC2_YEAST] Involved in the first step of pre-mRNA splicing. Required for cell growth and cell cycle control. Plays a role in the levels of the U1, U4, U5 and U6 snRNAs and the maintenance of the U4/U6 snRNA complex. May provide the link between the "nineteen complex" NTC spliceosome protein complex and the spliceosome through the U6 snRNA. Associates predominantly with U6 snRNAs in assembled active spliceosomes. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Binds also to U1, U4, U5 and U6 snRNAs and to pre-mRNAs, in vitro. Is not required for the Prp2-mediated remodeling of the activated spliceosome.[1] [2]
About this StructureAbout this Structure
3u1m is a 1 chain structure with sequence from Baker's yeast. Full crystallographic information is available from OCA.
ReferenceReference
- ↑ Lu P, Lu G, Yan C, Wang L, Li W, Yin P. Structure of the mRNA splicing complex component Cwc2: insights into RNA recognition. Biochem J. 2012 Jan 15;441(2):591-7. doi: 10.1042/BJ20111385. PMID:21957909 doi:http://dx.doi.org/10.1042/BJ20111385
- ↑ McGrail JC, Krause A, O'Keefe RT. The RNA binding protein Cwc2 interacts directly with the U6 snRNA to link the nineteen complex to the spliceosome during pre-mRNA splicing. Nucleic Acids Res. 2009 Jul;37(13):4205-17. Epub 2009 May 12. PMID:19435883 doi:http://dx.doi.org/gkp341
- ↑ Rasche N, Dybkov O, Schmitzova J, Akyildiz B, Fabrizio P, Luhrmann R. Cwc2 and its human homologue RBM22 promote an active conformation of the spliceosome catalytic centre. EMBO J. 2012 Mar 21;31(6):1591-604. doi: 10.1038/emboj.2011.502. Epub 2012 Jan, 13. PMID:22246180 doi:http://dx.doi.org/10.1038/emboj.2011.502