2m45: Difference between revisions
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{{STRUCTURE_2m45| PDB=2m45 | SCENE= }} | |||
===NMR solution structure of the C-terminus of the minichromosome maintenance protein MCM from Sulfolobus solfataricus=== | |||
The | ==Function== | ||
[[http://www.uniprot.org/uniprot/MCM_SULSO MCM_SULSO]] Presumptive replicative helicase. Has ATPase and DNA helicase activities. The latter preferentially melts 5'-tailed oligonucleotides and is stimulated by the SSB protein (single-stranded DNA binding protein). The active ATPase sites in the MCM ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The helicase function is proposed to use a partially sequential mode of ATP hydrolysis; the complex appears to tolerate multiple catalytically inactive subunits.<ref>PMID:11821426</ref> | |||
==About this Structure== | |||
[[2m45]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M45 OCA]. | |||
==Reference== | |||
<references group="xtra"/><references/> | |||
[[Category: DNA helicase]] | |||
[[Category: Gorlach, M.]] | |||
[[Category: Medagli, B.]] | |||
[[Category: Ohlenschlager, O.]] | |||
[[Category: Onesti, S.]] | |||
[[Category: Wiedemann, C.]] | |||
[[Category: Dna helicase]] | |||
[[Category: Helix-turn-helix]] | |||
[[Category: Hydrolase]] | |||
[[Category: Mcm]] | |||
[[Category: Minichromosome maintenance protein]] | |||
[[Category: Pre-replicative complex]] | |||
[[Category: Replication]] | |||
[[Category: Thermophile protein]] | |||
[[Category: Winged helix]] | |||
[[Category: Winged helix turn]] | |||
Revision as of 10:41, 29 January 2014
NMR solution structure of the C-terminus of the minichromosome maintenance protein MCM from Sulfolobus solfataricusNMR solution structure of the C-terminus of the minichromosome maintenance protein MCM from Sulfolobus solfataricus
FunctionFunction
[MCM_SULSO] Presumptive replicative helicase. Has ATPase and DNA helicase activities. The latter preferentially melts 5'-tailed oligonucleotides and is stimulated by the SSB protein (single-stranded DNA binding protein). The active ATPase sites in the MCM ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The helicase function is proposed to use a partially sequential mode of ATP hydrolysis; the complex appears to tolerate multiple catalytically inactive subunits.[1]
About this StructureAbout this Structure
2m45 is a 1 chain structure. Full experimental information is available from OCA.
ReferenceReference
- ↑ Carpentieri F, De Felice M, De Falco M, Rossi M, Pisani FM. Physical and functional interaction between the mini-chromosome maintenance-like DNA helicase and the single-stranded DNA binding protein from the crenarchaeon Sulfolobus solfataricus. J Biol Chem. 2002 Apr 5;277(14):12118-27. Epub 2002 Jan 30. PMID:11821426 doi:10.1074/jbc.M200091200