2yyi: Difference between revisions

Revision as of 19:58, 21 February 2008

Crystal structure of the oxygenase component (HpaB) of 4-hydroxyphenylacetate 3-monooxygenase complexed with FAD

OverviewOverview

The 4-hydroxyphenylacetate (4HPA) 3-monooxygenase is involved in the initial step of the 4HPA degradation pathway and catalyzes 4HPA hydroxylation to 3,4-dihydroxyphenylacetate. This enzyme consists of two components, an oxygenase (HpaB) and a reductase (HpaC). To understand the structural basis of the catalytic mechanism of HpaB, crystal structures of HpaB from Thermus thermophilus HB8 were determined in three states: a ligand-free form, a binary complex with FAD, and a ternary complex with FAD and 4HPA. Structural analysis revealed that the binding and dissociation of flavin are accompanied by conformational changes of the loop between beta5 and beta6 and of the loop between beta8 and beta9, leading to preformation of part of the substrate-binding site (Ser-197 and Thr-198). The latter loop further changes its conformation upon binding of 4HPA and obstructs the active site from the bulk solvent. Arg-100 is located adjacent to the putative oxygen-binding site and may be involved in the formation and stabilization of the C4a-hydroperoxyflavin intermediate.

About this StructureAbout this Structure

2YYI is a Single protein structure of sequence from Thermus thermophilus with and as ligands. Active as 4-hydroxyphenylacetate 3-monooxygenase, with EC number 1.14.13.3 Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the oxygenase component (HpaB) of the 4-hydroxyphenylacetate 3-monooxygenase from Thermus thermophilus HB8., Kim SH, Hisano T, Takeda K, Iwasaki W, Ebihara A, Miki K, J Biol Chem. 2007 Nov 9;282(45):33107-17. Epub 2007 Sep 5. PMID:17804419

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 ==Overview==
-The 4-hydroxyphenylacetate (4HPA) 3-monooxygenase is involved in the, initial step of the 4HPA degradation pathway and catalyzes 4HPA, hydroxylation to 3,4-dihydroxyphenylacetate. This enzyme consists of two, components, an oxygenase (HpaB) and a reductase (HpaC). To understand the, structural basis of the catalytic mechanism of HpaB, crystal structures of, HpaB from Thermus thermophilus HB8 were determined in three states: a, ligand-free form, a binary complex with FAD, and a ternary complex with, FAD and 4HPA. Structural analysis revealed that the binding and, dissociation of flavin are accompanied by conformational changes of the, loop between beta5 and beta6 and of the loop between beta8 and beta9, leading to preformation of part of the substrate-binding site (Ser-197 and, Thr-198). The latter loop further changes its conformation upon binding of, 4HPA and obstructs the active site from the bulk solvent. Arg-100 is, located adjacent to the putative oxygen-binding site and may be involved, in the formation and stabilization of the C4a-hydroperoxyflavin, intermediate.
+The 4-hydroxyphenylacetate (4HPA) 3-monooxygenase is involved in the initial step of the 4HPA degradation pathway and catalyzes 4HPA hydroxylation to 3,4-dihydroxyphenylacetate. This enzyme consists of two components, an oxygenase (HpaB) and a reductase (HpaC). To understand the structural basis of the catalytic mechanism of HpaB, crystal structures of HpaB from Thermus thermophilus HB8 were determined in three states: a ligand-free form, a binary complex with FAD, and a ternary complex with FAD and 4HPA. Structural analysis revealed that the binding and dissociation of flavin are accompanied by conformational changes of the loop between beta5 and beta6 and of the loop between beta8 and beta9, leading to preformation of part of the substrate-binding site (Ser-197 and Thr-198). The latter loop further changes its conformation upon binding of 4HPA and obstructs the active site from the bulk solvent. Arg-100 is located adjacent to the putative oxygen-binding site and may be involved in the formation and stabilization of the C4a-hydroperoxyflavin intermediate.
 ==About this Structure==
@@ Line 10: / Line 10: @@
 ==Reference==
-Crystal Structure of the Oxygenase Component (HpaB) of the 4-Hydroxyphenylacetate 3-Monooxygenase from Thermus thermophilus HB8., Kim SH, Hisano T, Takeda K, Iwasaki W, Ebihara A, Miki K, J Biol Chem. 2007 Nov 9;282(45):33107-17. Epub 2007 Sep 5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17804419 17804419]
+Crystal structure of the oxygenase component (HpaB) of the 4-hydroxyphenylacetate 3-monooxygenase from Thermus thermophilus HB8., Kim SH, Hisano T, Takeda K, Iwasaki W, Ebihara A, Miki K, J Biol Chem. 2007 Nov 9;282(45):33107-17. Epub 2007 Sep 5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17804419 17804419]
 [[Category: 4-hydroxyphenylacetate 3-monooxygenase]]
 [[Category: Single protein]]
@@ Line 17: / Line 17: @@
 [[Category: Hisano, T.]]
 [[Category: Iwasaki, W.]]
-[[Category: Kim, S.H.]]
+[[Category: Kim, S H.]]
 [[Category: Miki, K.]]
 [[Category: Takeda, K.]]
 [[Category: FAD]]
 [[Category: SO4]]
-[[Category: 4-hydroxyphenylacetate 3-monooxygenase]]
 [[Category: fad complex]]
 [[Category: oxidoreductase]]
@@ Line 30: / Line 29: @@
 [[Category: two-component flavin diffusible monooxygenase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:07:38 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:58:45 2008''